{"id":"b1f20a15-a4f1-5af3-af87-844a7b166f79","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-endothelium-c9","predicate":"inhibits","statement":"At 100 micromolar, c9,t11 inhibited endothelial eicosanoid production, whereas t10,c12 stimulated it.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2ac18d13-b49b-5c56-9d44-fdeecf94d273","mechanism_event_label":"Isomer and concentration changed the direction of the response.","subject":{"id":"a85c068b-9c0b-5a08-bad7-66ff3ef2af19","slug":"cla-cis9-trans11","display_name":"cis-9,trans-11 conjugated linoleic acid / rumenic acid","entity_type_key":"small_molecule"},"object":{"id":"eae36e04-83a2-57b8-b7dc-1eb0c6d20a47","slug":"human-endothelial-prostaglandin-output","display_name":"Human endothelial prostaglandin output","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2ac18d13-b49b-5c56-9d44-fdeecf94d273","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-endothelium-c9-event","event_type":"observed_relationship","label":"Isomer and concentration changed the direction of the response.","description":"At 100 micromolar, c9,t11 inhibited endothelial eicosanoid production, whereas t10,c12 stimulated it.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a85c068b-9c0b-5a08-bad7-66ff3ef2af19","slug":"cla-cis9-trans11","display_name":"cis-9,trans-11 conjugated linoleic acid / rumenic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"eae36e04-83a2-57b8-b7dc-1eb0c6d20a47","slug":"human-endothelial-prostaglandin-output","display_name":"Human endothelial prostaglandin output","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"39f9446f-52a6-502d-b507-91137726f82c","slug":"arachidonic-acid","display_name":"AA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human saphenous-vein endothelial cells with calcium-ionophore stimulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Lower-concentration results differed; no universal anti-inflammatory label.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Isomer and concentration changed the direction of the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The effect of conjugated linoleic acid on arachidonic acid metabolism and eicosanoid production in human saphenous vein endothelial cells. · 2002 · https://pubmed.ncbi.nlm.nih.gov/11880240/ · DOI 10.1016/s1388-1981(01)00198-6","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b8c3720d-7a4e-5d22-943a-c5ea56388859","evidence_kind":"source_excerpt","locator":"Lines 294-300","start_line":294,"end_line":300,"excerpt":"## cla-endothelium-c9\nIsomer and concentration changed the direction of the response.\nAt 100 micromolar, c9,t11 inhibited endothelial eicosanoid production, whereas t10,c12 stimulated it.\nModel: Human saphenous-vein endothelial cells with calcium-ionophore stimulation.\nLimitations: Lower-concentration results differed; no universal anti-inflammatory label.\nEvidence access: Primary abstract\nThe effect of conjugated linoleic acid on arachidonic acid metabolism and eicosanoid production in human saphenous vein endothelial cells. · 2002 · https://pubmed.ncbi.nlm.nih.gov/11880240/ · DOI 10.1016/s1388-1981(01)00198-6","model_system":"Human saphenous-vein endothelial cells with calcium-ionophore stimulation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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