{"id":"b1c324df-5a94-562b-bfeb-4f5a93048e0b","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-aldh9-activity-distribution","predicate":"has_substrate_activity_for","statement":"ALDH9/E3 betaine-aldehyde activity was highest in liver, adrenal and kidney among the tested tissues; mRNA peaked in different tissues.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"de91867f-1191-5d6f-9229-99d7faae9749","mechanism_event_label":"The enzyme’s measured activity did not simply track its RNA abundance.","subject":{"id":"c31af957-2ab3-5b80-b389-8989755433e4","slug":"aldh9a1","display_name":"ALDH9A1","entity_type_key":"protein"},"object":{"id":"2922542d-d263-5f46-afde-503b30e4940d","slug":"betaine-aldehyde","display_name":"Betaine aldehyde","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"de91867f-1191-5d6f-9229-99d7faae9749","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-aldh9-activity-distribution-event","event_type":"observed_intervention","label":"The enzyme’s measured activity did not simply track its RNA abundance.","description":"ALDH9/E3 betaine-aldehyde activity was highest in liver, adrenal and kidney among the tested tissues; mRNA peaked in different tissues.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c31af957-2ab3-5b80-b389-8989755433e4","slug":"aldh9a1","display_name":"ALDH9A1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2922542d-d263-5f46-afde-503b30e4940d","slug":"betaine-aldehyde","display_name":"Betaine aldehyde","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/9417993.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"67df6eb3c096ba09a314314ada97fc4c2b9ad555b28b316df3d17501b397c7d4\", \"start_char\": 0, \"end_char\": 941, \"text_sha256\": \"67df6eb3c096ba09a314314ada97fc4c2b9ad555b28b316df3d17501b397c7d4\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Tissue enzyme activity, immunoblot and RNA comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Betaine-aldehyde activity compared with E3/ALDH9 protein and transcript","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transcript abundance differed from protein/activity distribution; RNA is not a universal proxy for flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Choline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The enzyme’s measured activity did not simply track its RNA abundance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[choline-p9417993] Tissue distribution of human aldehyde dehydrogenase E3 (ALDH9): comparison of enzyme activity with E3 protein and mRNA distribution. (1997). https://pubmed.ncbi.nlm.nih.gov/9417993/ DOI: 10.1016/s0305-0491(97)00022-9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Multiple tissues including liver, adrenal, kidney and muscle","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ff0136c7-9f9b-515d-889f-062c14a4f4f0","evidence_kind":"source_excerpt","locator":"Lines 620-631","start_line":620,"end_line":631,"excerpt":"### choline-aldh9-activity-distribution\nALDH9/E3 betaine-aldehyde activity was highest in liver, adrenal and kidney among the tested tissues; mRNA peaked in different tissues.\nCondition category: normal\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The enzyme’s measured activity did not simply track its RNA abundance.\norganism: Human\ntissue_or_cell_type: Multiple tissues including liver, adrenal, kidney and muscle\nexperimental_model: Tissue enzyme activity, immunoblot and RNA comparison\nlimitations: Transcript abundance differed from protein/activity distribution; RNA is not a universal proxy for flux.\nexposure: Betaine-aldehyde activity compared with E3/ALDH9 protein and transcript\nevidence_span: {\"source_cache\": \"artifacts/choline-research/9417993.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"67df6eb3c096ba09a314314ada97fc4c2b9ad555b28b316df3d17501b397c7d4\", \"start_char\": 0, \"end_char\": 941, \"text_sha256\": \"67df6eb3c096ba09a314314ada97fc4c2b9ad555b28b316df3d17501b397c7d4\"}\n[choline-p9417993] Tissue distribution of human aldehyde dehydrogenase E3 (ALDH9): comparison of enzyme activity with E3 protein and mRNA distribution. 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