{"id":"b1bab133-06d4-5df8-9fab-93c39c1b8014","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-proline-respiration","predicate":"supports","statement":"Isolated mitochondria from human ZR75-30 breast cancer cells consumed oxygen with proline as the sole added substrate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d1ee6467-e827-5aa0-aa71-4059a68de788","mechanism_event_label":"In this model, proline can help fuel mitochondrial respiration.","subject":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"object":{"id":"5a4e28b0-ceac-54f5-a3ce-c91d470db7c1","slug":"human-zr7530-proline-respiration","display_name":"Proline-supported respiration in human ZR75-30 mitochondria","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d1ee6467-e827-5aa0-aa71-4059a68de788","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-proline-respiration-event","event_type":"observed_relationship","label":"In this model, proline can help fuel mitochondrial respiration.","description":"Isolated mitochondria from human ZR75-30 breast cancer cells consumed oxygen with proline as the sole added substrate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5a4e28b0-ceac-54f5-a3ce-c91d470db7c1","slug":"human-zr7530-proline-respiration","display_name":"Proline-supported respiration in human ZR75-30 mitochondria","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5e13a254-f92e-5317-abb5-2c683b178d39","slug":"prodh","display_name":"Human proline dehydrogenase / PRODH","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated human cancer-cell mitochondria.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Fuel use in this model does not quantify whole-body energy contribution or show a supplement benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"In this model, proline can help fuel mitochondrial respiration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Sources of superoxide/H2O2 during mitochondrial proline oxidation. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25184115/ · DOI 10.1016/j.redox.2014.07.003","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fd1c1914-5c35-53c7-962e-3b6012c065e4","evidence_kind":"source_excerpt","locator":"Lines 134-140","start_line":134,"end_line":140,"excerpt":"## l-proline-proline-respiration\nIn this model, proline can help fuel mitochondrial respiration.\nIsolated mitochondria from human ZR75-30 breast cancer cells consumed oxygen with proline as the sole added substrate.\nModel: Isolated human cancer-cell mitochondria.\nLimitations: Fuel use in this model does not quantify whole-body energy contribution or show a supplement benefit.\nEvidence access: Primary full text\nSources of superoxide/H2O2 during mitochondrial proline oxidation. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25184115/ · DOI 10.1016/j.redox.2014.07.003","model_system":"Isolated human cancer-cell mitochondria.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e5aa7fc5-ee52-5376-8169-416082a89fd1","stable_key":"import-6612c190-1948-5bcf-bbe3-a7f6c50fa3cf","title":"L-Proline: synthesis, collagen processing, redox metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7b7f1981f9c7897fbb6baa19425bdc19dede78ad4d66fa554394b8337c7366fb","revision_id":"4971a925-fa70-59f8-8030-d3029a18a62f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}