{"id":"b1a7c424-02a6-508f-87ed-ab2c318ab2d9","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-gcn2-chop","predicate":"required_for_induction","statement":"GCN2 loss prevented induction of stress-target genes such as CHOP even though amino-acid-limited CD8 cells still arrested.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ad0ee0d5-818d-5521-9bfb-8b5e0271804d","mechanism_event_label":"A stress gene response and the division checkpoint could be separated.","subject":{"id":"3876a24a-f6fe-5900-8ce2-22525a4faacb","slug":"mouse-eif2ak4","display_name":"Mouse GCN2 / Eif2ak4","entity_type_key":"protein"},"object":{"id":"8500f167-d0d5-547c-89bb-03484d03a666","slug":"mouse-ddit3","display_name":"Mouse CHOP / Ddit3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ad0ee0d5-818d-5521-9bfb-8b5e0271804d","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-gcn2-chop-event","event_type":"observed_relationship","label":"A stress gene response and the division checkpoint could be separated.","description":"GCN2 loss prevented induction of stress-target genes such as CHOP even though amino-acid-limited CD8 cells still arrested.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3876a24a-f6fe-5900-8ce2-22525a4faacb","slug":"mouse-eif2ak4","display_name":"Mouse GCN2 / Eif2ak4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8500f167-d0d5-547c-89bb-03484d03a666","slug":"mouse-ddit3","display_name":"Mouse CHOP / Ddit3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse Gcn2-deficient CD8 T-cell experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not make GCN2 dispensable for T-cell fitness; proliferation and trafficking defects were also observed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A stress gene response and the division checkpoint could be separated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Stress Kinase GCN2 Controls the Proliferative Fitness and Trafficking of Cytotoxic T Cells Independent of Environmental Amino Acid Sensing. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27880901/ · DOI 10.1016/j.celrep.2016.10.079","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2881eea7-d2f0-5748-aa63-c30bbc749aa0","evidence_kind":"source_excerpt","locator":"Lines 386-392","start_line":386,"end_line":392,"excerpt":"## tryptophan-gcn2-chop\nA stress gene response and the division checkpoint could be separated.\nGCN2 loss prevented induction of stress-target genes such as CHOP even though amino-acid-limited CD8 cells still arrested.\nModel: Mouse Gcn2-deficient CD8 T-cell experiments.\nLimitations: Does not make GCN2 dispensable for T-cell fitness; proliferation and trafficking defects were also observed.\nEvidence access: Primary abstract\nStress Kinase GCN2 Controls the Proliferative Fitness and Trafficking of Cytotoxic T Cells Independent of Environmental Amino Acid Sensing. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27880901/ · DOI 10.1016/j.celrep.2016.10.079","model_system":"Mouse Gcn2-deficient CD8 T-cell experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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