{"id":"b19491ca-11b0-5427-83e1-60045531d0f9","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:alliinase-localization","predicate":"reported_relationship","statement":"Alliinase localization to garlic bundle-sheath cells qualifies where precursor-enzyme mixing occurs after tissue damage.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"e4cdc2ee-197c-58a6-bd5f-efac1c1aae52","mechanism_event_label":"Alliinase localization to garlic bundle-sheath cells qualifies where precursor-enzyme mixing occurs after tissue damage.","subject":{"id":"30ba5e07-d4f1-5e89-8aa7-084f7ab9d7a7","slug":"garlic-alliinase","display_name":"Allium sativum alliin lyase / alliinase","entity_type_key":"protein"},"object":{"id":"663e293b-bb5a-5087-a547-049a1c82ab2f","slug":"alliin","display_name":"Alliin / (+S)-S-allyl-L-cysteine sulfoxide","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"e4cdc2ee-197c-58a6-bd5f-efac1c1aae52","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:alliinase-localization-event","event_type":"experimental_observation","label":"Alliinase localization to garlic bundle-sheath cells qualifies where precursor-enzyme mixing occurs after tissue damage.","description":"**Plant anatomy.** Alliinase was strongly localized to garlic-clove bundle-sheath cells, particularly around phloem, with little detected in storage mesophyll away from vascular bundles. Tissue disruption permits precursor–enzyme contact, but a uniform arrangement in every garlic cell is an oversimplification. Intact garlic still contains active metabolism and other constituents. [Ellmore and Feldberg 1994](https://bsapubs.onlinelibrary.wiley.com/doi/abs/10.1002/j.1537-2197.1994.tb15413.x)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"30ba5e07-d4f1-5e89-8aa7-084f7ab9d7a7","slug":"garlic-alliinase","display_name":"Allium sativum alliin lyase / alliinase","entity_type_key":"protein"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"663e293b-bb5a-5087-a547-049a1c82ab2f","slug":"alliin","display_name":"Alliin / (+S)-S-allyl-L-cysteine sulfoxide","entity_type_key":"small_molecule"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Publisher abstract read through web tool; anatomical localization, not independent full-section microscopy reanalysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Garlic-clove anatomical staining; primary publisher abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Spatial localization, not a causal inhibition or universal intracellular arrangement. Precursor is contextual; no human enzyme is implied.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Alliinase localization to garlic bundle-sheath cells qualifies where precursor-enzyme mixing occurs after tissue damage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Alliin lyase localization in bundle sheaths of the garlic clove (Allium sativum) | 1994 | DOI 10.1002/j.1537-2197.1994.tb15413.x | https://bsapubs.onlinelibrary.wiley.com/doi/abs/10.1002/j.1537-2197.1994.tb15413.x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 13-13; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5ce759d7-d17f-5112-81c1-4ea0d4d44883","evidence_kind":"source_excerpt","locator":"Lines 13-13","start_line":13,"end_line":13,"excerpt":"**Plant anatomy.** Alliinase was strongly localized to garlic-clove bundle-sheath cells, particularly around phloem, with little detected in storage mesophyll away from vascular bundles. Tissue disruption permits precursor–enzyme contact, but a uniform arrangement in every garlic cell is an oversimplification. Intact garlic still contains active metabolism and other constituents. [Ellmore and Feldberg 1994](https://bsapubs.onlinelibrary.wiley.com/doi/abs/10.1002/j.1537-2197.1994.tb15413.x)","model_system":"Garlic-clove anatomical staining; primary publisher abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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