{"id":"b1873e6a-5b4d-5305-bad0-a312fe4d66bc","stable_key":"e1af6d55-ced5-56c9-9a2d-8cc2ae6ec3ff:nasunin-viscosus-mic","predicate":"inhibited","statement":"The nasunin MIC for A. viscosus was 500 micrograms/mL.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4e0604ac-0471-59f5-b343-0d4a4ca3fa9b","mechanism_event_label":"A third tested species was inhibited.","subject":{"id":"6553997f-f098-5507-95b4-0fdb8aac491d","slug":"nasunin","display_name":"Nasunin","entity_type_key":"small_molecule"},"object":{"id":"67be86d6-ac75-5c03-9141-46aab97d5d94","slug":"a-viscosus-growth","display_name":"Actinomyces viscosus growth","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4e0604ac-0471-59f5-b343-0d4a4ca3fa9b","stable_key":"e1af6d55-ced5-56c9-9a2d-8cc2ae6ec3ff:nasunin-viscosus-mic-event","event_type":"biochemical_relationship","label":"A third tested species was inhibited.","description":"The nasunin MIC for A. viscosus was 500 micrograms/mL.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6553997f-f098-5507-95b4-0fdb8aac491d","slug":"nasunin","display_name":"Nasunin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"67be86d6-ac75-5c03-9141-46aab97d5d94","slug":"a-viscosus-growth","display_name":"Actinomyces viscosus growth","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/nasunin-research/dental2010.publisher-abstract.txt\", \"locator\": \"Primary publisher abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d369feccf2a98d02ce5f11168e967be5f9e95b51d02e45468a4e468e99c9d9b\", \"start_char\": 0, \"end_char\": 2042, \"text_sha256\": \"7d369feccf2a98d02ce5f11168e967be5f9e95b51d02e45468a4e468e99c9d9b\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Growth, resting-cell and crude-enzyme assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Nasunin MIC 500, 250 and 500 micrograms/mL respectively","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"In vitro concentrations and crude-enzyme preparations do not establish human caries prevention; glucosyltransferase isoforms were not resolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Nasunin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"nasunin","display_name":"Nasunin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Streptococcus mutans, Streptococcus sobrinus and Actinomyces viscosus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A third tested species was inhibited.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[nasunin-pdental2010] Antibacterial Effects of Nasunin against Cariogenic Bacteria (2010). https://www.jstage.jst.go.jp/article/shikahozon/53/3/53_KJ00006714377/_article/-char/en DOI: 10.11471/shikahozon.53.296","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Bacterial viability and extracellular glucan production","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"46936121-b581-57ac-8b20-8281ab0533c7","evidence_kind":"source_excerpt","locator":"Lines 1017-1028","start_line":1017,"end_line":1028,"excerpt":"### nasunin-viscosus-mic\nThe nasunin MIC for A. viscosus was 500 micrograms/mL.\nCondition category: normal\nnutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A third tested species was inhibited.\norganism: Streptococcus mutans, Streptococcus sobrinus and Actinomyces viscosus\ntissue_or_cell_type: Bacterial viability and extracellular glucan production\nexperimental_model: Growth, resting-cell and crude-enzyme assays\nlimitations: In vitro concentrations and crude-enzyme preparations do not establish human caries prevention; glucosyltransferase isoforms were not resolved.\nexposure: Nasunin MIC 500, 250 and 500 micrograms/mL respectively\nevidence_span: {\"source_cache\": \"artifacts/nasunin-research/dental2010.publisher-abstract.txt\", \"locator\": \"Primary publisher abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d369feccf2a98d02ce5f11168e967be5f9e95b51d02e45468a4e468e99c9d9b\", \"start_char\": 0, \"end_char\": 2042, \"text_sha256\": \"7d369feccf2a98d02ce5f11168e967be5f9e95b51d02e45468a4e468e99c9d9b\"}\n[nasunin-pdental2010] Antibacterial Effects of Nasunin against Cariogenic Bacteria (2010). https://www.jstage.jst.go.jp/article/shikahozon/53/3/53_KJ00006714377/_article/-char/en DOI: 10.11471/shikahozon.53.296","model_system":"Growth, resting-cell and crude-enzyme assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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