{"id":"b145ac2c-69bb-5ce2-8c15-48cee74b6dc4","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-gabat","predicate":"decreases_in_recorded_comparison","statement":"RA inhibited rat-brain GABA-transaminase activity by about 40% at 100 micrograms/mL, approximately 278 micromolar.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"7db0ae16-fc95-5792-958b-b6a50c88053e","mechanism_event_label":"RA inhibited rat-brain GABA-transaminase activity by about 40% at 100 micrograms/mL, approximately 278 micromolar.","subject":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"object":{"id":"1c5adbfc-d425-575a-9b09-c8eb647c8af7","slug":"rat-abat","display_name":"Rat GABA aminotransferase / ABAT","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"7db0ae16-fc95-5792-958b-b6a50c88053e","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-gabat-event","event_type":"experimental_observation","label":"RA inhibited rat-brain GABA-transaminase activity by about 40% at 100 micrograms/mL, approximately 278 micromolar.","description":"### ra-gabat\n\nRA inhibited rat-brain GABA-transaminase activity by about 40% at 100 micrograms/mL, approximately 278 micromolar.\nUrsolic and oleanolic acids were also active constituents; human brain exposure and GABA elevation were not established.\nModel and exposure: Bioassay-guided lemon-balm fractionation; enzyme assay.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Bioassay-guided fractionation of lemon balm (Melissa officinalis L.) using an in vitro measure of GABA transaminase activity. | 2009 | https://pubmed.ncbi.nlm.nih.gov/19165747/ | https://doi.org/10.1002/ptr.2712 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Purified RA at 100 micrograms/mL","sequence_order":0,"notes":""},{"entity":{"id":"1c5adbfc-d425-575a-9b09-c8eb647c8af7","slug":"rat-abat","display_name":"Rat GABA aminotransferase / ABAT","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"d5b98fa8-0612-5a5a-aa3c-6cfd8ff88867","slug":"gaba","display_name":"Gamma-aminobutyric acid","entity_type_key":"small_molecule"},"role":"enzyme substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Purified RA at 100 micrograms/mL\", \"comparator\": \"No inhibitor\", \"endpoint\": \"RA inhibited rat-brain GABA-transaminase activity by about 40% at 100 micrograms/mL, approximately 278 micromolar.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Bioassay-guided lemon-balm fractionation; enzyme assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"RA inhibited rat-brain GABA-transaminase activity by about 40% at 100 micrograms/mL, approximately 278 micromolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Bioassay-guided fractionation of lemon balm (Melissa officinalis L.) using an in vitro measure of GABA transaminase activity. | https://pubmed.ncbi.nlm.nih.gov/19165747/ | https://doi.org/10.1002/ptr.2712","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-gabat; Ursolic and oleanolic acids were also active constituents; human brain exposure and GABA elevation were not established.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"815e03a7-dcb5-5ac2-9b28-3eca84829f44","evidence_kind":"source_excerpt","locator":"Lines 441-447","start_line":441,"end_line":447,"excerpt":"### ra-gabat\n\nRA inhibited rat-brain GABA-transaminase activity by about 40% at 100 micrograms/mL, approximately 278 micromolar.\nUrsolic and oleanolic acids were also active constituents; human brain exposure and GABA elevation were not established.\nModel and exposure: Bioassay-guided lemon-balm fractionation; enzyme assay.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Bioassay-guided fractionation of lemon balm (Melissa officinalis L.) using an in vitro measure of GABA transaminase activity. | 2009 | https://pubmed.ncbi.nlm.nih.gov/19165747/ | https://doi.org/10.1002/ptr.2712 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"Bioassay-guided lemon-balm fractionation; enzyme assay.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}