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Results: Increased circulating 1,25(OH)2D levels impair mineralization by upregulating mineralization inhibitors","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"D3 active metabolite or VDR machinery experiment; not a direct D2-versus-D3 comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Differentiated primary Vdr-intact mouse osteoblasts; conditioned-medium pyrophosphate normalized to DNA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"10^-8 M calcitriol for 4 days after osteogenic differentiation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Direct culture result does not imply that usual dietary vitamin D damages bone; physiological outcome also depends on intestinal mineral delivery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin D2 and D3","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; 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(2012). https://pubmed.ncbi.nlm.nih.gov/22523068/ DOI: 10.1172/jci45890","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Osteoblast culture","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"65d8c38b-ea87-5794-a800-8551c7045938","evidence_kind":"source_excerpt","locator":"Lines 809-823","start_line":809,"end_line":823,"excerpt":"### vdm-calcitriol-increases-extracellular-ppi\nCalcitriol treatment increased extracellular pyrophosphate in differentiated Vdr-intact mouse osteoblast cultures.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: In this model, active vitamin D increased a molecule that restrains mineral deposition.\norganism: Mus musculus\ntissue_or_cell_type: Osteoblast culture\nexperimental_model: Differentiated primary Vdr-intact mouse osteoblasts; conditioned-medium pyrophosphate normalized to DNA\nlimitations: Direct culture result does not imply that usual dietary vitamin D damages bone; physiological outcome also depends on intestinal mineral delivery.\nexposure: 10^-8 M calcitriol for 4 days after osteogenic differentiation.\ncross_nutrient: Vitamin D–calcium–phosphate regulation.\nevidence_locator: Figure 6; Results: Increased circulating 1,25(OH)2D levels impair mineralization by upregulating mineralization inhibitors\nnutrient: Vitamin D2 and D3\nevidence_scope: D3 active metabolite or VDR machinery experiment; not a direct D2-versus-D3 comparison.\n[vdm-lieben2012] Normocalcemia is maintained in mice under conditions of calcium malabsorption by vitamin D-induced inhibition of bone mineralization. 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