{"id":"b111cb27-65a9-58eb-aff3-176915e36703","stable_key":"649e861b-265a-5912-bc36-3a73e53ef892:l-serine-glycine-racemase","predicate":"inhibits","statement":"Intracellular glycine tonically inhibited serine racemase in the study, while glycine exposure could transiently promote D-serine release through Asc-1.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"05307545-c715-5183-9be1-2bad653d1eec","mechanism_event_label":"The same neighboring amino acid can affect both production and release in different ways.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"ad4f7e6e-2d43-5d8b-9b79-24b8a65777c6","slug":"mouse-srr","display_name":"Mouse serine racemase / Srr","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"05307545-c715-5183-9be1-2bad653d1eec","stable_key":"649e861b-265a-5912-bc36-3a73e53ef892:l-serine-glycine-racemase-event","event_type":"observed_relationship","label":"The same neighboring amino acid can affect both production and release in different ways.","description":"Intracellular glycine tonically inhibited serine racemase in the study, while glycine exposure could transiently promote D-serine release through Asc-1.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ad4f7e6e-2d43-5d8b-9b79-24b8a65777c6","slug":"mouse-srr","display_name":"Mouse serine racemase / Srr","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7f3bf9f5-b1b1-5381-baa6-72800ceb5436","slug":"d-serine","display_name":"D-Serine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"1c585303-7ccc-5207-86ce-e7c62adbc88f","slug":"mouse-slc7a10","display_name":"Mouse Asc-1 amino-acid transporter / Slc7a10","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Biochemical assays, primary cultures and mouse microdialysis; Asc-1 knockout abolished the release response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Inhibition of synthesis and stimulation of release are distinct endpoints, not contradictory directions for one reaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Serine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The same neighboring amino acid can affect both production and release in different ways.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The NMDA receptor activation by d-serine and glycine is controlled by an astrocytic Phgdh-dependent serine shuttle. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31548413/ · DOI 10.1073/pnas.1909458116","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"75f20382-5eee-5db3-ab60-ec7744c141f2","evidence_kind":"source_excerpt","locator":"Lines 358-364","start_line":358,"end_line":364,"excerpt":"## l-serine-glycine-racemase\nThe same neighboring amino acid can affect both production and release in different ways.\nIntracellular glycine tonically inhibited serine racemase in the study, while glycine exposure could transiently promote D-serine release through Asc-1.\nModel: Biochemical assays, primary cultures and mouse microdialysis; Asc-1 knockout abolished the release response.\nLimitations: Inhibition of synthesis and stimulation of release are distinct endpoints, not contradictory directions for one reaction.\nEvidence access: Primary abstract\nThe NMDA receptor activation by d-serine and glycine is controlled by an astrocytic Phgdh-dependent serine shuttle. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31548413/ · DOI 10.1073/pnas.1909458116","model_system":"Biochemical assays, primary cultures and mouse microdialysis; Asc-1 knockout abolished the release response.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"70bcf57a-36bb-563f-9073-2d616e5f155a","stable_key":"import-649e861b-265a-5912-bc36-3a73e53ef892","title":"L-Serine: synthesis, one-carbon metabolism, lipids and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"22a750ce2b94f8607268c58322c48c4f0468f403b72a4008307538a6e5ff86d5","revision_id":"c1d6a7e1-9558-5bc6-9e3b-7080c26d3dbc","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}