{"id":"b0f7737f-ebb5-53bf-bd65-68f7db0d963f","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-kmo-loss-nad-preserved","predicate":"does-not-necessarily-determine","statement":"Measured liver and brain NAD+ contents were not significantly different between Kmo-knockout and wild-type mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"aee596be-84c2-5ef0-92bc-b6ef0f4503cf","mechanism_event_label":"Losing the KMO branch did not collapse the measured NAD+ pools.","subject":{"id":"41e2c985-04a2-503e-a39a-91bdcc0403f3","slug":"mouse-kmo","display_name":"Mouse kynurenine 3-monooxygenase / Kmo","entity_type_key":"protein"},"object":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"aee596be-84c2-5ef0-92bc-b6ef0f4503cf","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-kmo-loss-nad-preserved-event","event_type":"biochemical_relationship","label":"Losing the KMO branch did not collapse the measured NAD+ pools.","description":"Measured liver and brain NAD+ contents were not significantly different between Kmo-knockout and wild-type mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"measured tissue pool","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"41e2c985-04a2-503e-a39a-91bdcc0403f3","slug":"mouse-kmo","display_name":"Mouse kynurenine 3-monooxygenase / Kmo","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Bounds the B2-KMO-niacin link: a cofactor-dependent pathway step is not proof that total NAD pools must fall.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Results: liver Fig 4 and brain Fig 7; matching abstract conclusion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Constitutive Kmo knockout and wild-type mice, approximately two months old; liver and brain metabolite assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Constitutive Kmo deletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Steady-state pools do not measure de novo synthesis flux; diet and salvage can affect the result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Losing the KMO branch did not collapse the measured NAD+ pools.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[giorgini2013] Targeted deletion of kynurenine 3-monooxygenase in mice: a new tool for studying kynurenine pathway metabolism in periphery and brain. (2013). https://pubmed.ncbi.nlm.nih.gov/24189070/ DOI: 10.1074/jbc.m113.503813","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver and brain","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"a1614163-65c8-54f8-9463-03386bfdb6b7","evidence_kind":"source_excerpt","locator":"Lines 1262-1274","start_line":1262,"end_line":1274,"excerpt":"### b2-kmo-loss-nad-preserved\nMeasured liver and brain NAD+ contents were not significantly different between Kmo-knockout and wild-type mice.\nCondition category: machinery_impairment\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Losing the KMO branch did not collapse the measured NAD+ pools.\norganism: Mus musculus\ntissue_or_cell_type: Liver and brain\nexperimental_model: Constitutive Kmo knockout and wild-type mice, approximately two months old; liver and brain metabolite assays.\nlimitations: Steady-state pools do not measure de novo synthesis flux; diet and salvage can affect the result.\nexposure: Constitutive Kmo deletion\ncross_nutrient: Bounds the B2-KMO-niacin link: a cofactor-dependent pathway step is not proof that total NAD pools must fall.\nevidence_location: Results: liver Fig 4 and brain Fig 7; matching abstract conclusion\n[giorgini2013] Targeted deletion of kynurenine 3-monooxygenase in mice: a new tool for studying kynurenine pathway metabolism in periphery and brain. (2013). https://pubmed.ncbi.nlm.nih.gov/24189070/ DOI: 10.1074/jbc.m113.503813","model_system":"Constitutive Kmo knockout and wild-type mice, approximately two months old; liver and brain metabolite assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [giorgini2013] Targeted deletion of kynurenine 3-monooxygenase in mice: a new tool for studying kynurenine pathway metabolism in periphery and brain. (2013). https://pubmed.ncbi.nlm.nih.gov/24189070/ DOI: 10.1074/jbc.m113.503813","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"680cb6bc8249877f2410f551807f10d390fdd719014137d7414ba3420e29228d","revision_id":"7a61e299-908d-5372-860b-99ed190f9d7a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}