{"id":"b0d9b8a1-959f-5137-a75c-74056cade0b0","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-ketoacid-feedback","predicate":"inhibits","statement":"2-Oxobutyrate inhibited BCOADC kinase, and reduced BCOADC phosphorylation was observed in isolated adipocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"5b290dad-ea27-522c-8fa3-21939f259b3a","mechanism_event_label":"A catabolic intermediate can influence the regulatory brake on its disposal machinery.","subject":{"id":"dee03e61-050d-5bde-ad0a-7f9652799ec8","slug":"alpha-ketobutyrate","display_name":"Alpha-ketobutyrate","entity_type_key":"small_molecule"},"object":{"id":"967dacbf-b001-56cb-9df3-b797e5588170","slug":"study-1986-bcoadc-kinase","display_name":"BCOADC kinase in the 1986 study, source species unverified","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"5b290dad-ea27-522c-8fa3-21939f259b3a","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-ketoacid-feedback-event","event_type":"observed_relationship","label":"A catabolic intermediate can influence the regulatory brake on its disposal machinery.","description":"2-Oxobutyrate inhibited BCOADC kinase, and reduced BCOADC phosphorylation was observed in isolated adipocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee03e61-050d-5bde-ad0a-7f9652799ec8","slug":"alpha-ketobutyrate","display_name":"Alpha-ketobutyrate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"967dacbf-b001-56cb-9df3-b797e5588170","slug":"study-1986-bcoadc-kinase","display_name":"BCOADC kinase in the 1986 study, source species unverified","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a57dc55e-a10a-5cba-9728-5f95f141a583","slug":"study-1986-purified-bcoadc","display_name":"Purified BCOADC in the 1986 ketoacid study, source species unverified","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified kinase and isolated-adipocyte assays; species unverified from abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This does not prove that a threonine supplement changes BCAA oxidation in humans. Correction record: PubMed indexes an erratum in Biochemical Journal 1987;242(3):935. The notice text was inaccessible during this curation; its specific scope and impact remain unverified. The purified-enzyme findings are retained provisionally with this flag, and the abstract does not identify the source species. https://pubmed.ncbi.nlm.nih.gov/3800905/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A catabolic intermediate can influence the regulatory brake on its disposal machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Oxidative decarboxylation of 4-methylthio-2-oxobutyrate by branched-chain 2-oxo acid dehydrogenase complex. · 1986 · https://pubmed.ncbi.nlm.nih.gov/3800905/ · DOI 10.1042/bj2370621","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"14892c9b-17be-56ca-bf8e-f4a66d51c2c7","evidence_kind":"source_excerpt","locator":"Lines 314-320","start_line":314,"end_line":320,"excerpt":"## l-threonine-ketoacid-feedback\nA catabolic intermediate can influence the regulatory brake on its disposal machinery.\n2-Oxobutyrate inhibited BCOADC kinase, and reduced BCOADC phosphorylation was observed in isolated adipocytes.\nModel: Purified kinase and isolated-adipocyte assays; species unverified from abstract.\nLimitations: This does not prove that a threonine supplement changes BCAA oxidation in humans. Correction record: PubMed indexes an erratum in Biochemical Journal 1987;242(3):935. The notice text was inaccessible during this curation; its specific scope and impact remain unverified. The purified-enzyme findings are retained provisionally with this flag, and the abstract does not identify the source species. https://pubmed.ncbi.nlm.nih.gov/3800905/\nEvidence access: Primary abstract\nOxidative decarboxylation of 4-methylthio-2-oxobutyrate by branched-chain 2-oxo acid dehydrogenase complex. · 1986 · https://pubmed.ncbi.nlm.nih.gov/3800905/ · DOI 10.1042/bj2370621","model_system":"Purified kinase and isolated-adipocyte assays; species unverified from abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3365aed939d9f567bd098449c7d67c7c00fb6c9ba163dcf34dc7cf6081f324fb","revision_id":"10a7b648-8dd7-5d4e-b618-c560a6e2d3c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}