{"id":"b0d487c6-e914-57d9-872a-91963068b54c","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-ppa-viability","predicate":"reduces","statement":"Purpurazoic acid reduced HEK293-cell viability with a reported IC50 of 78 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"46dd5a04-c004-5649-889a-e5a40379db36","mechanism_event_label":"Purpurazoic acid reduced HEK293-cell viability with a reported IC50 of 78 micromolar.","subject":{"id":"c24c43c0-a4cf-548f-987a-c0ac4738ff29","slug":"purpurazoic-acid","display_name":"Purpurazoic acid / PPA","entity_type_key":"small_molecule"},"object":{"id":"e1f3bb8f-1b9a-523b-94ed-23cfcd0df2f0","slug":"human-hek293-viability","display_name":"Viability of human HEK293 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"46dd5a04-c004-5649-889a-e5a40379db36","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-ppa-viability-event","event_type":"observed_relationship","label":"Purpurazoic acid reduced HEK293-cell viability with a reported IC50 of 78 micromolar.","description":"Purpurazoic acid reduced HEK293-cell viability with a reported IC50 of 78 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c24c43c0-a4cf-548f-987a-c0ac4738ff29","slug":"purpurazoic-acid","display_name":"Purpurazoic acid / PPA","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e1f3bb8f-1b9a-523b-94ed-23cfcd0df2f0","slug":"human-hek293-viability","display_name":"Viability of human HEK293 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"SCAP and PPA concentration series; IC50 values 89 and 78 micromolar respectively","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Viability exposure duration not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract; unrecovered method details explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HEK293 cells exposed to chemically characterized metabolites","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"These concentrations concern isolated metabolites, not parent tartrazine, and do not establish human dietary toxicity or cancer risk.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human HEK293 cells exposed to chemically characterized metabolites","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Purpurazoic acid reduced HEK293-cell viability with a reported IC50 of 78 micromolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Preparation, analysis and toxicity characterisation of the redox metabolites of the azo food dye tartrazine. (2023). https://pubmed.ncbi.nlm.nih.gov/37980979/ DOI: 10.1016/j.fct.2023.114193","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro metabolite addition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Cell viability assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"42acf9fd-16e8-58af-8272-25cdc871fbfc","evidence_kind":"source_excerpt","locator":"Lines 127-136","start_line":127,"end_line":136,"excerpt":"## tartrazine-ppa-viability\nPurpurazoic acid reduced HEK293-cell viability with a reported IC50 of 78 micromolar.\nModel/species: Human HEK293 cells exposed to chemically characterized metabolites\nTissue: Cell viability assay\nExposure: SCAP and PPA concentration series; IC50 values 89 and 78 micromolar respectively\nRoute: In vitro metabolite addition\nDuration: Viability exposure duration not specified in accessed abstract\nLimits: These concentrations concern isolated metabolites, not parent tartrazine, and do not establish human dietary toxicity or cancer risk.\nPrimary reference: Preparation, analysis and toxicity characterisation of the redox metabolites of the azo food dye tartrazine. (2023). https://pubmed.ncbi.nlm.nih.gov/37980979/ DOI: 10.1016/j.fct.2023.114193\nAccess: Primary PubMed abstract; unrecovered method details explicitly retained.","model_system":"Human HEK293 cells exposed to chemically characterized metabolites","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. 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