{"id":"b0d3b453-9b21-5422-bd35-22ae9dbaf6c3","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-hela-bax","predicate":"increases_expression_of","statement":"Coumarin increased BAX expression in HeLa cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fc281391-8a1b-5e03-8ea7-53e4c688741d","mechanism_event_label":"A protein associated with mitochondrial apoptosis increased.","subject":{"id":"9fd9b93a-fdfd-5e09-92d3-0495bff41391","slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"},"object":{"id":"c2d61996-e216-593f-9621-71aba2448649","slug":"bax","display_name":"Human BAX","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"fc281391-8a1b-5e03-8ea7-53e4c688741d","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-hela-bax-event","event_type":"biochemical_relationship","label":"A protein associated with mitochondrial apoptosis increased.","description":"Coumarin increased BAX expression in HeLa cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9fd9b93a-fdfd-5e09-92d3-0495bff41391","slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c2d61996-e216-593f-9621-71aba2448649","slug":"bax","display_name":"Human BAX","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coumarin-research/18210747.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e78df713d6bc2e34f13e80a0ef04c217a176b5d6082cbb28fa5a62464fcce842\", \"start_char\": 0, \"end_char\": 1237, \"text_sha256\": \"e78df713d6bc2e34f13e80a0ef04c217a176b5d6082cbb28fa5a62464fcce842\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell viability, cell cycle, protein and mitochondrial assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Coumarin; reported viability IC50 54.2 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cultured cancer-cell response does not establish treatment efficacy; indexed abstract does not specify all exposure durations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coumarin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human cell line","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A protein associated with mitochondrial apoptosis increased.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coumarin-p18210747] Coumarin induces cell cycle arrest and apoptosis in human cervical cancer HeLa cells through a mitochondria- and caspase-3 dependent mechanism and NF-kappaB down-regulation. (2007). https://pubmed.ncbi.nlm.nih.gov/18210747/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HeLa cervical cancer cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b7213e73-62c5-5c64-9b82-ff1780082fb4","evidence_kind":"source_excerpt","locator":"Lines 371-382","start_line":371,"end_line":382,"excerpt":"### coumarin-hela-bax\nCoumarin increased BAX expression in HeLa cells.\nCondition category: normal\nnutrient_topic: Coumarin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A protein associated with mitochondrial apoptosis increased.\norganism: Human cell line\ntissue_or_cell_type: HeLa cervical cancer cells\nexperimental_model: Cell viability, cell cycle, protein and mitochondrial assays\nlimitations: Cultured cancer-cell response does not establish treatment efficacy; indexed abstract does not specify all exposure durations.\nexposure: Coumarin; reported viability IC50 54.2 micromolar\nevidence_span: {\"source_cache\": \"artifacts/coumarin-research/18210747.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e78df713d6bc2e34f13e80a0ef04c217a176b5d6082cbb28fa5a62464fcce842\", \"start_char\": 0, \"end_char\": 1237, \"text_sha256\": \"e78df713d6bc2e34f13e80a0ef04c217a176b5d6082cbb28fa5a62464fcce842\"}\n[coumarin-p18210747] Coumarin induces cell cycle arrest and apoptosis in human cervical cancer HeLa cells through a mitochondria- and caspase-3 dependent mechanism and NF-kappaB down-regulation. 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