{"id":"b0d086ac-81c2-5e50-a425-bb7b1bdc8b8f","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-oct1","predicate":"transports","statement":"Human OCT1 expression increased concentration-dependent agmatine transport in HEK293 cells, with Km 18.73 ± 4.86 mM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f9cf80e7-4c04-5824-a4a2-742330f8b909","mechanism_event_label":"A transporter can admit the molecule, but its affinity matters.","subject":{"id":"3cced77c-b044-5d97-8c81-4ff9ed3dd169","slug":"slc22a1","display_name":"Human organic cation transporter 1 / OCT1 / SLC22A1","entity_type_key":"protein"},"object":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"f9cf80e7-4c04-5824-a4a2-742330f8b909","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-oct1-event","event_type":"observed_relationship","label":"A transporter can admit the molecule, but its affinity matters.","description":"Human OCT1 expression increased concentration-dependent agmatine transport in HEK293 cells, with Km 18.73 ± 4.86 mM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3cced77c-b044-5d97-8c81-4ff9ed3dd169","slug":"slc22a1","display_name":"Human organic cation transporter 1 / OCT1 / SLC22A1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human transporter expressed in HEK293 cells; radiolabeled agmatine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"An in vitro Km is not a target blood concentration or oral bioavailability estimate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A transporter can admit the molecule, but its affinity matters.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"OCT2 and MATE1 provide bidirectional agmatine transport. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21128598/ · DOI 10.1021/mp100180a","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Expression increased concentration-dependent agmatine transport into HEK293 cells.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Expression increased concentration-dependent agmatine transport into HEK293 cells.","entity":null}],"evidence":[{"id":"f8bf9983-c7be-5d91-a1bd-ee6827be116e","evidence_kind":"source_excerpt","locator":"Lines 20-26","start_line":20,"end_line":26,"excerpt":"## agmatine-sulfate-oct1\nA transporter can admit the molecule, but its affinity matters.\nHuman OCT1 expression increased concentration-dependent agmatine transport in HEK293 cells, with Km 18.73 ± 4.86 mM.\nModel: Human transporter expressed in HEK293 cells; radiolabeled agmatine.\nLimitations: An in vitro Km is not a target blood concentration or oral bioavailability estimate.\nEvidence access: Primary abstract\nOCT2 and MATE1 provide bidirectional agmatine transport. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21128598/ · DOI 10.1021/mp100180a","model_system":"Human transporter expressed in HEK293 cells; radiolabeled agmatine.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b55d70e22d07b80964f825770d92fdd1745221420ed8af8d94ca5182b9994509","revision_id":"fc7be029-20e0-5943-b74e-3b06ba694571","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}