{"id":"b0a95552-1fa5-5129-bd66-affac904fbfc","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-i-polymerization","predicate":"inhibits_tested","statement":"Adding cucurbitacin I inhibited actin polymerization in the 2024 in-vitro experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f166e2e8-fee6-504b-bb1c-269722d73629","mechanism_event_label":"Filament assembly is a separate endpoint from filament breakdown.","subject":{"id":"55ef0ba6-495d-5cc4-88c5-0772ab25d0fe","slug":"cucurbitacin-i","display_name":"Cucurbitacin I","entity_type_key":"small_molecule"},"object":{"id":"3a1ca6e1-3a53-55cf-afc0-ed0dfdce4b7f","slug":"purified-actin-polymerization-cui-2024","display_name":"Purified actin polymerization in the 2024 cucurbitacin I assay","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f166e2e8-fee6-504b-bb1c-269722d73629","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-i-polymerization-event","event_type":"observed_relationship","label":"Filament assembly is a separate endpoint from filament breakdown.","description":"Adding cucurbitacin I inhibited actin polymerization in the 2024 in-vitro experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"55ef0ba6-495d-5cc4-88c5-0772ab25d0fe","slug":"cucurbitacin-i","display_name":"Cucurbitacin I","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3a1ca6e1-3a53-55cf-afc0-ed0dfdce4b7f","slug":"purified-actin-polymerization-cui-2024","display_name":"Purified actin polymerization in the 2024 cucurbitacin I assay","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7633cd49-57aa-5817-b6e9-f26a4334ca42","slug":"purified-cucurbitacin-g-actin","display_name":"Purified G-actin in cucurbitacin assays, species as reported","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified actin plus endothelial-cell experiments; cell species not resolved in this abstract-based record.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Concentration-dependent and assay-dependent findings require matched replication before declaring a resolved direct-target disagreement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Filament assembly is a separate endpoint from filament breakdown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Depolymerization of actin filaments by Cucurbitacin I through binding G-actin. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38370084/ · DOI 10.1002/fsn3.3804","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f351c071-e535-5487-bfeb-16b3fefadd18","evidence_kind":"source_excerpt","locator":"Lines 92-98","start_line":92,"end_line":98,"excerpt":"## cucurbitacin-i-polymerization\nFilament assembly is a separate endpoint from filament breakdown.\nAdding cucurbitacin I inhibited actin polymerization in the 2024 in-vitro experiment.\nModel: Purified actin plus endothelial-cell experiments; cell species not resolved in this abstract-based record.\nLimitations: Concentration-dependent and assay-dependent findings require matched replication before declaring a resolved direct-target disagreement.\nEvidence access: Primary abstract\nDepolymerization of actin filaments by Cucurbitacin I through binding G-actin. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38370084/ · DOI 10.1002/fsn3.3804","model_system":"Purified actin plus endothelial-cell experiments; cell species not resolved in this abstract-based record.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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