{"id":"b07dfb75-4ae2-551c-8f3d-2805f7b23499","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-fpgs-cytosolic-trapping","predicate":"supports","statement":"Human cytosolic FPGS reconstitution supported persistent folate pools in AUXB1 cells; labeled folate loss was not measurable over three cell generations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"5dae7032-2fc4-5549-87c3-6336e1bea065","mechanism_event_label":"Cytosolic tail-building helped keep folate inside cells.","subject":{"id":"ef23c0b4-93bc-5f74-9889-cb7e573568a6","slug":"fpgs-cytosolic","display_name":"Human cytosolic FPGS isoform","entity_type_key":"protein"},"object":{"id":"e9560075-8968-5455-8244-31f8ab75ad1e","slug":"cellular-folate-retention","display_name":"Cellular folate retention","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5dae7032-2fc4-5549-87c3-6336e1bea065","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-fpgs-cytosolic-trapping-event","event_type":"biochemical_relationship","label":"Cytosolic tail-building helped keep folate inside cells.","description":"Human cytosolic FPGS reconstitution supported persistent folate pools in AUXB1 cells; labeled folate loss was not measurable over three cell generations.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"ef23c0b4-93bc-5f74-9889-cb7e573568a6","slug":"fpgs-cytosolic","display_name":"Human cytosolic FPGS isoform","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e9560075-8968-5455-8244-31f8ab75ad1e","slug":"cellular-folate-retention","display_name":"Cellular folate retention","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"81c45abb-713d-521f-9854-23288f880831","slug":"folate-polyglutamates","display_name":"Folate polyglutamates","entity_type_key":"chemical_species"},"role":"retained pool","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human FPGS isoforms in Chinese hamster AUXB1 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Doxycycline-induced cytosolic FPGS and tracer chase","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Non-detection applies to the tested cell model and observation window.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human enzyme in Cricetulus griseus cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Cytosolic tail-building helped keep folate inside cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lawrence2014] Mammalian mitochondrial and cytosolic folylpolyglutamate synthetase maintain the subcellular compartmentalization of folates (2014). https://pubmed.ncbi.nlm.nih.gov/25164808/ DOI: 10.1074/jbc.m114.593244","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"AUXB1 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f979e61f-8ca0-5123-90ec-017d9faf8085","evidence_kind":"source_excerpt","locator":"Lines 279-289","start_line":279,"end_line":289,"excerpt":"### folate-fpgs-cytosolic-trapping\nHuman cytosolic FPGS reconstitution supported persistent folate pools in AUXB1 cells; labeled folate loss was not measurable over three cell generations.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Cytosolic tail-building helped keep folate inside cells.\norganism: Human enzyme in Cricetulus griseus cells\ntissue_or_cell_type: AUXB1 cells\nexperimental_model: Human FPGS isoforms in Chinese hamster AUXB1 cells\nlimitations: Non-detection applies to the tested cell model and observation window.\nexposure: Doxycycline-induced cytosolic FPGS and tracer chase\n[lawrence2014] Mammalian mitochondrial and cytosolic folylpolyglutamate synthetase maintain the subcellular compartmentalization of folates (2014). https://pubmed.ncbi.nlm.nih.gov/25164808/ DOI: 10.1074/jbc.m114.593244","model_system":"Human FPGS isoforms in Chinese hamster AUXB1 cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [lawrence2014] Mammalian mitochondrial and cytosolic folylpolyglutamate synthetase maintain the subcellular compartmentalization of folates (2014). https://pubmed.ncbi.nlm.nih.gov/25164808/ DOI: 10.1074/jbc.m114.593244","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}