{"id":"b052bf22-0fce-5c3d-9090-906cd4808b2c","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-copper-nifedipine","predicate":"enhances_myricetin_associated","statement":"L-type calcium-channel blockade with 10 micromolar nifedipine exacerbated death during copper/myricetin exposure.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c5074c6e-b324-5b4a-bc07-8cc671d95a3c","mechanism_event_label":"Changing calcium entry altered the response.","subject":{"id":"1c59d504-7042-5922-b484-0819e3b9a93d","slug":"nifedipine","display_name":"Nifedipine","entity_type_key":"small_molecule"},"object":{"id":"9fe548f7-074d-50a1-857e-759828b72d55","slug":"human-shsy5y-copper-cell-death","display_name":"Human SH-SY5Y death under copper exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c5074c6e-b324-5b4a-bc07-8cc671d95a3c","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-copper-nifedipine-event","event_type":"observed_relationship","label":"Changing calcium entry altered the response.","description":"L-type calcium-channel blockade with 10 micromolar nifedipine exacerbated death during copper/myricetin exposure.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1c59d504-7042-5922-b484-0819e3b9a93d","slug":"nifedipine","display_name":"Nifedipine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9fe548f7-074d-50a1-857e-759828b72d55","slug":"human-shsy5y-copper-cell-death","display_name":"Human SH-SY5Y death under copper exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human SH-SY5Y pharmacological perturbation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Channel subtype and causal downstream steps were not resolved; no human medication interaction established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Changing calcium entry altered the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Neurotoxic Effect of Flavonol Myricetin in the Presence of Excess Copper. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33562817/ · DOI 10.3390/molecules26040845","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"08c581f7-fabc-5db3-84ad-73088087bcfb","evidence_kind":"source_excerpt","locator":"Lines 364-370","start_line":364,"end_line":370,"excerpt":"## myricetin-copper-nifedipine\nChanging calcium entry altered the response.\nL-type calcium-channel blockade with 10 micromolar nifedipine exacerbated death during copper/myricetin exposure.\nModel: Human SH-SY5Y pharmacological perturbation.\nLimitations: Channel subtype and causal downstream steps were not resolved; no human medication interaction established.\nEvidence access: Primary full text\nNeurotoxic Effect of Flavonol Myricetin in the Presence of Excess Copper. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33562817/ · DOI 10.3390/molecules26040845","model_system":"Human SH-SY5Y pharmacological perturbation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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