{"id":"b02acfb2-5968-5ef7-910a-4ff05131925c","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-microbial-atp","predicate":"produces","statement":"E. coli TdcD or AckA converted propionyl phosphate to propionate with ATP generation in the anaerobic threonine-degradation pathway.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"78098153-423a-59b4-bd7c-7cdd2a7848a8","mechanism_event_label":"A microbial fermentation step releases energy as well as a short-chain fatty acid.","subject":{"id":"bb66c39c-a6ff-5e85-91aa-5409da205b51","slug":"e-coli-tdcd","display_name":"Escherichia coli propionate kinase / TdcD","entity_type_key":"protein"},"object":{"id":"f3eec97e-b0aa-59a2-9947-b205a7efdb10","slug":"propionate","display_name":"Propionate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"78098153-423a-59b4-bd7c-7cdd2a7848a8","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-microbial-atp-event","event_type":"observed_relationship","label":"A microbial fermentation step releases energy as well as a short-chain fatty acid.","description":"E. coli TdcD or AckA converted propionyl phosphate to propionate with ATP generation in the anaerobic threonine-degradation pathway.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bb66c39c-a6ff-5e85-91aa-5409da205b51","slug":"e-coli-tdcd","display_name":"Escherichia coli propionate kinase / TdcD","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f3eec97e-b0aa-59a2-9947-b205a7efdb10","slug":"propionate","display_name":"Propionate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5d16b350-67b9-548e-9fe7-c41bee412ae6","slug":"propionyl-phosphate","display_name":"Propionyl phosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"9676e061-29f5-5b34-bb17-0ac77e6468e1","slug":"e-coli-acka","display_name":"Escherichia coli acetate kinase / AckA","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mutant pathway analysis and enzyme characterization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Whether this pathway changes human host exposure depends on community, substrate supply and environmental conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A microbial fermentation step releases energy as well as a short-chain fatty acid.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Novel keto acid formate-lyase and propionate kinase enzymes are components of an anaerobic pathway in Escherichia coli that degrades L-threonine to propionate. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9484901/ · DOI 10.1046/j.1365-2958.1998.00696.x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c0e99523-777d-57d7-b6a3-8e1772f7e43e","evidence_kind":"source_excerpt","locator":"Lines 434-440","start_line":434,"end_line":440,"excerpt":"## l-threonine-microbial-atp\nA microbial fermentation step releases energy as well as a short-chain fatty acid.\nE. coli TdcD or AckA converted propionyl phosphate to propionate with ATP generation in the anaerobic threonine-degradation pathway.\nModel: Mutant pathway analysis and enzyme characterization.\nLimitations: Whether this pathway changes human host exposure depends on community, substrate supply and environmental conditions.\nEvidence access: Primary abstract\nNovel keto acid formate-lyase and propionate kinase enzymes are components of an anaerobic pathway in Escherichia coli that degrades L-threonine to propionate. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9484901/ · DOI 10.1046/j.1365-2958.1998.00696.x","model_system":"Mutant pathway analysis and enzyme characterization.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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