{"id":"b015047f-e1fd-51b2-921d-99e603d27798","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-mir146a","predicate":"increases_expression","statement":"Orthosilicic acid increased miR-146a during later osteogenic differentiation of human mesenchymal stromal cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"de1d5539-079f-5411-a7eb-a0d05f69c848","mechanism_event_label":"A regulatory RNA connects exposure to cell-state change.","subject":{"id":"c497144d-1e90-5176-a475-2d715992f5b0","slug":"orthosilicic-acid","display_name":"Orthosilicic acid / Si(OH)4","entity_type_key":"small_molecule"},"object":{"id":"8d309e8c-00a5-5788-a323-634f6d4de8d0","slug":"human-mir146a","display_name":"Human miR-146a","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"de1d5539-079f-5411-a7eb-a0d05f69c848","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-mir146a-event","event_type":"observed_relationship","label":"A regulatory RNA connects exposure to cell-state change.","description":"Orthosilicic acid increased miR-146a during later osteogenic differentiation of human mesenchymal stromal cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c497144d-1e90-5176-a475-2d715992f5b0","slug":"orthosilicic-acid","display_name":"Orthosilicic acid / Si(OH)4","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8d309e8c-00a5-5788-a323-634f6d4de8d0","slug":"human-mir146a","display_name":"Human miR-146a","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human MSC microarray and qPCR experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Late-stage association is distinct from identification of the upstream silicon sensor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A regulatory RNA connects exposure to cell-state change.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Orthosilicic acid, Si(OH)4, stimulates osteoblast differentiation in vitro by upregulating miR-146a to antagonize NF-κB activation. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27163405/ · DOI 10.1016/j.actbio.2016.05.007","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2c96db91-6a5e-5c96-93f8-c6cdf01c4deb","evidence_kind":"source_excerpt","locator":"Lines 184-190","start_line":184,"end_line":190,"excerpt":"## silica-mir146a\nA regulatory RNA connects exposure to cell-state change.\nOrthosilicic acid increased miR-146a during later osteogenic differentiation of human mesenchymal stromal cells.\nModel: Human MSC microarray and qPCR experiments.\nLimitations: Late-stage association is distinct from identification of the upstream silicon sensor.\nEvidence access: Primary abstract\nOrthosilicic acid, Si(OH)4, stimulates osteoblast differentiation in vitro by upregulating miR-146a to antagonize NF-κB activation. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27163405/ · DOI 10.1016/j.actbio.2016.05.007","model_system":"Human MSC microarray and qPCR experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"e05d81962a1ca6cbfc68fce4fd3dbcdea67c8e61332c19f4fec5d0c3ab8bf1b9","revision_id":"6433ed49-a331-571b-a611-21e56c3ce4bc","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}