{"id":"b000e5e8-6541-5fe6-97ec-87730eead6be","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-mouse-tdh-methylation","predicate":"when_depleted_reduces","statement":"Depleting Tdh also lowered SAM and H3K4 trimethylation in the mouse stem-cell experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"672b09a5-042b-574f-ad6c-3354b9bd14ed","mechanism_event_label":"The precursor requires an active metabolic pathway to affect the downstream mark.","subject":{"id":"9b018d12-5ae6-5c78-bd51-84c2bd7f18a7","slug":"mouse-tdh","display_name":"Mouse L-threonine dehydrogenase / Tdh","entity_type_key":"protein"},"object":{"id":"0761a2f7-7956-5109-a4c2-0b4c8de17773","slug":"mouse-esc-sam-h3k4me3","display_name":"Mouse embryonic stem-cell SAM and H3K4 trimethylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"672b09a5-042b-574f-ad6c-3354b9bd14ed","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-mouse-tdh-methylation-event","event_type":"observed_relationship","label":"The precursor requires an active metabolic pathway to affect the downstream mark.","description":"Depleting Tdh also lowered SAM and H3K4 trimethylation in the mouse stem-cell experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9b018d12-5ae6-5c78-bd51-84c2bd7f18a7","slug":"mouse-tdh","display_name":"Mouse L-threonine dehydrogenase / Tdh","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0761a2f7-7956-5109-a4c2-0b4c8de17773","slug":"mouse-esc-sam-h3k4me3","display_name":"Mouse embryonic stem-cell SAM and H3K4 trimethylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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unverified.","entity":null}],"evidence":[{"id":"5a97a6fa-b645-58ef-8797-aa7657ab2b45","evidence_kind":"source_excerpt","locator":"Lines 338-344","start_line":338,"end_line":344,"excerpt":"## l-threonine-mouse-tdh-methylation\nThe precursor requires an active metabolic pathway to affect the downstream mark.\nDepleting Tdh also lowered SAM and H3K4 trimethylation in the mouse stem-cell experiments.\nModel: Mouse embryonic stem-cell Tdh depletion, alongside dietary-substrate tracing in the same study.\nLimitations: The experiment and threonine withdrawal are complementary tests from one paper, not independent laboratories.\nEvidence access: Primary abstract\nInfluence of threonine metabolism on S-adenosylmethionine and histone methylation. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23118012/ · DOI 10.1126/science.1226603","model_system":"Mouse embryonic stem-cell Tdh depletion, alongside dietary-substrate tracing in the same study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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