{"id":"afad15a9-b5aa-58bc-8921-01b89ab01b9b","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gaba-current","predicate":"decreases_in_recorded_comparison","statement":"Apigenin decreased GABA-activated chloride currents in cultured cerebellar granule cells; flumazenil antagonized that effect.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"8c3c4bbe-5f5a-5d93-875e-259a042423bf","mechanism_event_label":"Apigenin decreased GABA-activated chloride currents in cultured cerebellar granule cells; flumazenil antagonized that effect.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"eab1a566-5195-5091-9780-6f6c779e3dda","slug":"apigenin-cerebellar-gaba-current","display_name":"GABA-activated chloride current in the apigenin cerebellar-cell assay","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8c3c4bbe-5f5a-5d93-875e-259a042423bf","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gaba-current-event","event_type":"experimental_observation","label":"Apigenin decreased GABA-activated chloride currents in cultured cerebellar granule cells; flumazenil antagonized that effect.","description":"### api-gaba-current\n\nApigenin decreased GABA-activated chloride currents in cultured cerebellar granule cells; flumazenil antagonized that effect.\nThis is not evidence of a conventional positive allosteric modulator. Chloride-current direction is not a blood chloride depletion claim.\nModel and exposure: Electrophysiology; cell species not specified in the accessed abstract.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Pharmacological profile of apigenin, a flavonoid isolated from Matricaria chamomilla. | 2000 | https://pubmed.ncbi.nlm.nih.gov/10751547/ | https://doi.org/10.1016/s0006-2952(00)00264-1 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Apigenin during GABA application","sequence_order":0,"notes":""},{"entity":{"id":"eab1a566-5195-5091-9780-6f6c779e3dda","slug":"apigenin-cerebellar-gaba-current","display_name":"GABA-activated chloride current in the apigenin cerebellar-cell assay","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"d5b98fa8-0612-5a5a-aa3c-6cfd8ff88867","slug":"gaba","display_name":"Gamma-aminobutyric acid","entity_type_key":"small_molecule"},"role":"agonist","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6a844eb8-a21b-54ed-8e57-9d830a16312b","slug":"chloride","display_name":"Chloride","entity_type_key":"nutrient_element"},"role":"conducted ion","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e06afc45-5686-53e3-ace8-da08e2d4dc7f","slug":"flumazenil","display_name":"Flumazenil / Ro 15-1788","entity_type_key":"drug"},"role":"blocking comparator","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"Apigenin during GABA application","comparator":"GABA response without apigenin","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"agonist applied during electrophysiology","comparator":"GABA response without apigenin","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"gaba","display_name":"Gamma-aminobutyric acid","entity_type_key":"small_molecule"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Apigenin during GABA application\", \"comparator\": \"GABA response without apigenin\", \"endpoint\": \"Apigenin decreased GABA-activated chloride currents in cultured cerebellar granule cells; flumazenil antagonized that effect.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"apigenin\", \"state\": \"Apigenin during GABA application\"}, {\"entity_slug\": \"gaba\", \"state\": \"agonist applied during electrophysiology\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Electrophysiology; cell species not specified in the accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Apigenin decreased GABA-activated chloride currents in cultured cerebellar granule cells; flumazenil antagonized that effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Pharmacological profile of apigenin, a flavonoid isolated from Matricaria chamomilla. | https://pubmed.ncbi.nlm.nih.gov/10751547/ | https://doi.org/10.1016/s0006-2952(00)00264-1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-gaba-current; This is not evidence of a conventional positive allosteric modulator. Chloride-current direction is not a blood chloride depletion claim.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"59802f60-128d-5aab-938a-b0e0a091baca","evidence_kind":"source_excerpt","locator":"Lines 788-794","start_line":788,"end_line":794,"excerpt":"### api-gaba-current\n\nApigenin decreased GABA-activated chloride currents in cultured cerebellar granule cells; flumazenil antagonized that effect.\nThis is not evidence of a conventional positive allosteric modulator. Chloride-current direction is not a blood chloride depletion claim.\nModel and exposure: Electrophysiology; cell species not specified in the accessed abstract.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Pharmacological profile of apigenin, a flavonoid isolated from Matricaria chamomilla. | 2000 | https://pubmed.ncbi.nlm.nih.gov/10751547/ | https://doi.org/10.1016/s0006-2952(00)00264-1 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"Electrophysiology; cell species not specified in the accessed abstract.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[{"id":"57bd48b8-b81a-5b87-b6a4-49f65263530c","relation_type":"limits_scope_of","notes":"Binding-site affinity does not determine the sign of functional modulation.","source_claim_id":"afad15a9-b5aa-58bc-8921-01b89ab01b9b","source_claim_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gaba-current","source_statement":"Apigenin decreased GABA-activated chloride currents in cultured cerebellar granule cells; flumazenil antagonized that effect.","target_claim_id":"16d9e93d-b071-53e0-ada2-7949494ade0d","target_claim_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gaba-binding","target_statement":"Apigenin displaced flunitrazepam binding with reported Ki 4 micromolar in the 1995 receptor-binding study."}],"conflicts":[],"corrections":[],"research":null}