{"id":"af5d31de-256d-5d40-a82c-bb820194d1e5","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-nicotine-inhibition","predicate":"inhibits_in_assay","statement":"Nicotine inhibited CYP2A6 coumarin hydroxylation within the study inhibitor category of Ki below 200 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"112f8d97-cf4f-5d46-8aae-bd171c253176","mechanism_event_label":"Nicotine and coumarin can interact in an enzyme assay.","subject":{"id":"22c6c620-aea4-55ec-9c2a-3cbf914b8997","slug":"nicotine","display_name":"Nicotine","entity_type_key":"small_molecule"},"object":{"id":"8a5084db-2b31-59a9-8afc-3ad0980d8f5d","slug":"coumarin-7-hydroxylation","display_name":"Coumarin 7-hydroxylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"112f8d97-cf4f-5d46-8aae-bd171c253176","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-nicotine-inhibition-event","event_type":"biochemical_relationship","label":"Nicotine and coumarin can interact in an enzyme assay.","description":"Nicotine inhibited CYP2A6 coumarin hydroxylation within the study inhibitor category of Ki below 200 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"22c6c620-aea4-55ec-9c2a-3cbf914b8997","slug":"nicotine","display_name":"Nicotine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8a5084db-2b31-59a9-8afc-3ad0980d8f5d","slug":"coumarin-7-hydroxylation","display_name":"Coumarin 7-hydroxylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coumarin-research/9143352.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"dca3f4253c2977d47070bb95696634ee2e06fa9a28a3d76ac9790ce695ed6f09\", \"start_char\": 0, \"end_char\": 2723, \"text_sha256\": \"dca3f4253c2977d47070bb95696634ee2e06fa9a28a3d76ac9790ce695ed6f09\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human microsomal and recombinant CYP2A6 inhibitor screen","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Coumarin 0.5–50 micromolar; 47 inhibitor/substrate probes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"In vitro inhibition thresholds are not demonstrated clinical interactions at food exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coumarin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Nicotine and coumarin can interact in an enzyme assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coumarin-p9143352] Inhibition of coumarin 7-hydroxylase activity in human liver microsomes. 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