{"id":"aef18b55-9b44-5bc1-98d6-dd0b46baffcb","stable_key":"e054f0f5-417f-5850-a874-91ff2c9de631:apt2-c2s-oe-dhhc6","predicate":"has_contextual_experimental_effect","statement":"Palmitoylation-deficient APT2-C2S overexpression did not reproduce the significant reduction in ZDHHC6 palmitoylation caused by wild-type APT2.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"c0bf193d-d725-5edd-8f98-94f8990e2d09","mechanism_event_label":"apt2 c2s oe dhhc6","subject":{"id":"5a6f23ef-b397-506f-9bf0-1bd11362b4b1","slug":"human-apt2-c2s-overexpression-hela","display_name":"Human APT2-C2S overexpression in HeLa","entity_type_key":"protein_state"},"object":{"id":"05e9ea97-39b9-5e0a-8dde-6c337bdd67dd","slug":"regulatory-palmitoylated-zdhhc6","display_name":"Regulatory palmitoylated ZDHHC6","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"c0bf193d-d725-5edd-8f98-94f8990e2d09","stable_key":"e054f0f5-417f-5850-a874-91ff2c9de631:apt2-c2s-oe-dhhc6","event_type":"experimental_result","label":"apt2 c2s oe dhhc6","description":"Palmitoylation-deficient APT2-C2S overexpression did not reproduce the significant reduction in ZDHHC6 palmitoylation caused by wild-type APT2.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5a6f23ef-b397-506f-9bf0-1bd11362b4b1","slug":"human-apt2-c2s-overexpression-hela","display_name":"Human APT2-C2S overexpression in HeLa","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"05e9ea97-39b9-5e0a-8dde-6c337bdd67dd","slug":"regulatory-palmitoylated-zdhhc6","display_name":"Regulatory palmitoylated ZDHHC6","entity_type_key":"protein_state"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f2ffef5b-5457-50a8-b787-b8f24430f610","slug":"lypla2","display_name":"LYPLA2","entity_type_key":"protein"},"role":"base protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a2fd4b35-5483-5e7c-9604-d792fa67d402","slug":"zdhhc6","display_name":"ZDHHC6","entity_type_key":"protein"},"role":"measured protein","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_cache","value_text":"artifacts/discovery-research/round6-sources/zdhhc6-primary-passages.json; SHA256 a02040bb8922a9170e095cdfb5e875a5e7f513b682c0ec69c19feacb3dc191b0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HeLa; tagged human APT2-C2S and ZDHHC6 constructs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"24-hour plasmid expression, then 2-hour tritiated-palmitate labeling; normalized to ZDHHC6 protein; n=6.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"These are separate experimental observations, not a demonstrated sulforaphane-GPX4-ZDHHC6 pathway. Sulforaphane-driven APT2 relocalization is not equivalent to genetic depletion or general catalytic inhibition. No dietary, clinical or selenium-repletion effect is inferred. Protein stability, palmitoylation and substrate output remain different measurements. This mutant result supports a role for APT2 palmitoylation in this substrate assay; it is not a sulforaphane experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human experimental cells; construct species unresolved where stated for discussion-only nulls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"Figure 2E,F; Rapid APT2-mediated ZDHHC6 depalmitoylation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.7554/eLife.27826","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_access","value_text":"Selected primary Results, figure legends and methods via indexed publisher text. 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No supplement or raw-data reanalysis.\nevidence_cache: artifacts/discovery-research/round6-sources/zdhhc6-primary-passages.json; SHA256 a02040bb8922a9170e095cdfb5e875a5e7f513b682c0ec69c19feacb3dc191b0\nexperimental_model: Human HeLa; tagged human APT2-C2S and ZDHHC6 constructs\norganism: Human experimental cells; construct species unresolved where stated for discussion-only nulls\nexposure: 24-hour plasmid expression, then 2-hour tritiated-palmitate labeling; normalized to ZDHHC6 protein; n=6.\nlimitations: These are separate experimental observations, not a demonstrated sulforaphane-GPX4-ZDHHC6 pathway. Sulforaphane-driven APT2 relocalization is not equivalent to genetic depletion or general catalytic inhibition. No dietary, clinical or selenium-repletion effect is inferred. Protein stability, palmitoylation and substrate output remain different measurements. This mutant result supports a role for APT2 palmitoylation in this substrate assay; it is not a sulforaphane experiment.","model_system":"Human HeLa; tagged human APT2-C2S and ZDHHC6 constructs","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase and primary locator; not a verbatim publisher quote. Null-result limitations retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d7785cbf-e87c-5662-a76c-7ef11586cca5","stable_key":"import-e054f0f5-417f-5850-a874-91ff2c9de631","title":"APT2: sulforaphane engagement, GPX4 stability and ZDHHC6 turnover","document_type":"imported_text","citation_label":"Targeted primary-literature curation from recursive ZDHHC6 exploration, 2026-09-20.","file_path":"","sha256":"011acbeafd419a6826789806b59b5d7ff312b222762824580036325083586956","revision_id":"663a0bd1-3e46-5fee-ab0f-dcf992b2a809","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}