{"id":"aeba999d-8b73-55d2-91da-a210881722fa","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-xct-stress","predicate":"increases_with_stress_alongside","statement":"Diethyl maleate induced xCT expression and increased cystine uptake in U87 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2be9e61e-267a-5ea1-b33a-0e3f7b631c67","mechanism_event_label":"The cells adjusted precursor uptake during the experimental challenge.","subject":{"id":"aac19cc3-1b68-5ca3-a896-f61184f7320a","slug":"slc7a11","display_name":"SLC7A11","entity_type_key":"protein"},"object":{"id":"609df3aa-110e-54b4-b072-985dc72c81b2","slug":"cystine-uptake","display_name":"cystine uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2be9e61e-267a-5ea1-b33a-0e3f7b631c67","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-xct-stress-event","event_type":"biochemical_relationship","label":"The cells adjusted precursor uptake during the experimental challenge.","description":"Diethyl maleate induced xCT expression and increased cystine uptake in U87 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"aac19cc3-1b68-5ca3-a896-f61184f7320a","slug":"slc7a11","display_name":"SLC7A11","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"609df3aa-110e-54b4-b072-985dc72c81b2","slug":"cystine-uptake","display_name":"cystine uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/glutathione-research/11406111.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e7118afd8c6055ed926ee24dbb7a07538f676163d605115ae0d32d49fbef7fdc\", \"start_char\": 0, \"end_char\": 1364, \"text_sha256\": \"e7118afd8c6055ed926ee24dbb7a07538f676163d605115ae0d32d49fbef7fdc\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human xCT expression and oxidative-stress response","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"hxCTb with 4F2hc; diethyl maleate challenge","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Sodium independence belongs to this transporter; other cysteine-entry routes differ.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glutathione research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human transporter; Xenopus expression host","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The cells adjusted precursor uptake during the experimental challenge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[glutathione-p11406111] Human cystine/glutamate transporter: cDNA cloning and upregulation by oxidative stress in glioma cells. (2001). https://pubmed.ncbi.nlm.nih.gov/11406111/ DOI: 10.1016/s0005-2736(01)00338-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Oocyte membranes and human U87 glioma cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"362af991-571e-5e6c-8bfb-af295c50bfe7","evidence_kind":"source_excerpt","locator":"Lines 749-760","start_line":749,"end_line":760,"excerpt":"### glutathione-xct-stress\nDiethyl maleate induced xCT expression and increased cystine uptake in U87 cells.\nCondition category: normal\nnutrient_topic: Glutathione research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The cells adjusted precursor uptake during the experimental challenge.\norganism: Human transporter; Xenopus expression host\ntissue_or_cell_type: Oocyte membranes and human U87 glioma cells\nexperimental_model: Human xCT expression and oxidative-stress response\nlimitations: Sodium independence belongs to this transporter; other cysteine-entry routes differ.\nexposure: hxCTb with 4F2hc; diethyl maleate challenge\nevidence_span: {\"source_cache\": \"artifacts/glutathione-research/11406111.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e7118afd8c6055ed926ee24dbb7a07538f676163d605115ae0d32d49fbef7fdc\", \"start_char\": 0, \"end_char\": 1364, \"text_sha256\": \"e7118afd8c6055ed926ee24dbb7a07538f676163d605115ae0d32d49fbef7fdc\"}\n[glutathione-p11406111] Human cystine/glutamate transporter: cDNA cloning and upregulation by oxidative stress in glioma cells. (2001). https://pubmed.ncbi.nlm.nih.gov/11406111/ DOI: 10.1016/s0005-2736(01)00338-8","model_system":"Human xCT expression and oxidative-stress response","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [glutathione-p11406111] Human cystine/glutamate transporter: cDNA cloning and upregulation by oxidative stress in glioma cells. (2001). https://pubmed.ncbi.nlm.nih.gov/11406111/ DOI: 10.1016/s0005-2736(01)00338-8","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9b7417fd-059d-5683-b452-71d1e3cf82d0","stable_key":"import-db0fc92e-b5ef-5667-a4c5-3ef257edbc9b","title":"Glutathione: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2a5740c4a7e770aafe883fea47909b18bf5e174f211749935130a50f9dea2753","revision_id":"a64016dd-4b72-5954-a72d-0e61de854073","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}