{"id":"ae98e47f-de5b-53bf-8a9e-b689c4f4ac24","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-acute-plasma-gsh-null","predicate":"did_not_change","statement":"Plasma glutathione did not change during the pilot’s three-hour sampling period.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"06039bea-c5fe-5444-af12-d5276f34c08e","mechanism_event_label":"An acute blood measurement did not reproduce every cell-culture glutathione result.","subject":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"object":{"id":"bc93e288-d973-5d91-b1fb-f183a12b1a55","slug":"plasma-glutathione-concentration-unspecified","display_name":"Plasma glutathione concentration, assay pool unspecified","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"06039bea-c5fe-5444-af12-d5276f34c08e","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-acute-plasma-gsh-null-event","event_type":"observed_intervention","label":"An acute blood measurement did not reproduce every cell-culture glutathione result.","description":"Plasma glutathione did not change during the pilot’s three-hour sampling period.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"measured_metabolite","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bc93e288-d973-5d91-b1fb-f183a12b1a55","slug":"plasma-glutathione-concentration-unspecified","display_name":"Plasma glutathione concentration, assay pool unspecified","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ala-research/22609537.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"bfd228f5a8102a99fecaf7c6de8d1cb910afacd695cb3947c60987f54da431fa\", \"start_char\": 0, \"end_char\": 1645, \"text_sha256\": \"bfd228f5a8102a99fecaf7c6de8d1cb910afacd695cb3947c60987f54da431fa\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Small crossover pharmacokinetic pilot in younger and older adults","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"500 mg R-lipoic acid versus 500 mg racemic lipoic acid","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The indexed abstract does not specify reduced versus total glutathione assay pool; retain that uncertainty. A three-hour blood measurement is not a test of all intracellular pools.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An acute blood measurement did not reproduce every cell-culture glutathione result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ala-p22609537] Age and gender dependent bioavailability of R- and R,S-α-lipoic acid: a pilot study. (2012). https://pubmed.ncbi.nlm.nih.gov/22609537/ DOI: 10.1016/j.phrs.2012.05.002","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Plasma","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"16a382df-d5b0-5326-b146-4b294e731ae0","evidence_kind":"source_excerpt","locator":"Lines 676-687","start_line":676,"end_line":687,"excerpt":"### ala-acute-plasma-gsh-null\nPlasma glutathione did not change during the pilot’s three-hour sampling period.\nCondition category: normal\nnutrient_topic: Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: An acute blood measurement did not reproduce every cell-culture glutathione result.\norganism: Human\ntissue_or_cell_type: Plasma\nexperimental_model: Small crossover pharmacokinetic pilot in younger and older adults\nlimitations: The indexed abstract does not specify reduced versus total glutathione assay pool; retain that uncertainty. A three-hour blood measurement is not a test of all intracellular pools.\nexposure: 500 mg R-lipoic acid versus 500 mg racemic lipoic acid\nevidence_span: {\"source_cache\": \"artifacts/ala-research/22609537.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"bfd228f5a8102a99fecaf7c6de8d1cb910afacd695cb3947c60987f54da431fa\", \"start_char\": 0, \"end_char\": 1645, \"text_sha256\": \"bfd228f5a8102a99fecaf7c6de8d1cb910afacd695cb3947c60987f54da431fa\"}\n[ala-p22609537] Age and gender dependent bioavailability of R- and R,S-α-lipoic acid: a pilot study. (2012). https://pubmed.ncbi.nlm.nih.gov/22609537/ DOI: 10.1016/j.phrs.2012.05.002","model_system":"Small crossover pharmacokinetic pilot in younger and older adults","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ala-p22609537] Age and gender dependent bioavailability of R- and R,S-α-lipoic acid: a pilot study. (2012). https://pubmed.ncbi.nlm.nih.gov/22609537/ DOI: 10.1016/j.phrs.2012.05.002","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d8afa8c2-ced9-5b28-90ca-2ac120ec7202","stable_key":"import-5d8e27d8-6a74-5560-827f-3f90908bbc34","title":"Alpha-lipoic acid: cofactor assembly, redox signaling and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"611da198ab85a272eb26b64ee330ef6d1a762853a8481f3191c4acfca9f3cf3d","revision_id":"618b4ce6-3157-5222-8325-408064be5ea5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}