{"id":"ae8bf904-202e-5258-bc86-c8eb5ab76a5a","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-impa-lithium","predicate":"inhibits","statement":"Li+ inhibited human inositol monophosphatase; parallel changes in Li+ and Mg2+ affinity across mutants supported a shared metal-binding site.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ed9e78ce-7504-526d-b5fd-e0d91504d82e","mechanism_event_label":"Lithium can interfere with a magnesium-linked step of inositol recycling.","subject":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"object":{"id":"3f404867-317e-5b5c-92a7-9b6e0411dec3","slug":"impa1","display_name":"IMPA1 (human inositol monophosphatase 1)","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ed9e78ce-7504-526d-b5fd-e0d91504d82e","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-impa-lithium-event","event_type":"biochemical_relationship","label":"Lithium can interfere with a magnesium-linked step of inositol recycling.","description":"Li+ inhibited human inositol monophosphatase; parallel changes in Li+ and Mg2+ affinity across mutants supported a shared metal-binding site.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3f404867-317e-5b5c-92a7-9b6e0411dec3","slug":"impa1","display_name":"IMPA1 (human inositol monophosphatase 1)","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/8223565.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"945d1d5166ce6410cd69984d93aebf127b635dcfcebddba31f0e8cc8b1c304a5\", \"start_char\": 0, \"end_char\": 1621, \"text_sha256\": \"945d1d5166ce6410cd69984d93aebf127b635dcfcebddba31f0e8cc8b1c304a5\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Site-directed mutagenesis and enzyme kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Magnesium and lithium titrations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Biochemical concentration dependence; neither lithium treatment mechanism nor magnesium supplementation response is established by this assay alone.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human recombinant protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Lithium can interfere with a magnesium-linked step of inositol recycling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p8223565] Probing the role of metal ions in the mechanism of inositol monophosphatase by site-directed mutagenesis. (1993). https://pubmed.ncbi.nlm.nih.gov/8223565/ DOI: 10.1111/j.1432-1033.1993.tb18244.x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified IMPA1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"93cda2af-f500-560b-b58e-761e170823f1","evidence_kind":"source_excerpt","locator":"Lines 275-286","start_line":275,"end_line":286,"excerpt":"### ino-impa-lithium\nLi+ inhibited human inositol monophosphatase; parallel changes in Li+ and Mg2+ affinity across mutants supported a shared metal-binding site.\nCondition category: machinery_impairment\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Lithium can interfere with a magnesium-linked step of inositol recycling.\norganism: Human recombinant protein\ntissue_or_cell_type: Purified IMPA1\nexperimental_model: Site-directed mutagenesis and enzyme kinetics\nlimitations: Biochemical concentration dependence; neither lithium treatment mechanism nor magnesium supplementation response is established by this assay alone.\nexposure: Magnesium and lithium titrations\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/8223565.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"945d1d5166ce6410cd69984d93aebf127b635dcfcebddba31f0e8cc8b1c304a5\", \"start_char\": 0, \"end_char\": 1621, \"text_sha256\": \"945d1d5166ce6410cd69984d93aebf127b635dcfcebddba31f0e8cc8b1c304a5\"}\n[ino-p8223565] Probing the role of metal ions in the mechanism of inositol monophosphatase by site-directed mutagenesis. (1993). https://pubmed.ncbi.nlm.nih.gov/8223565/ DOI: 10.1111/j.1432-1033.1993.tb18244.x","model_system":"Site-directed mutagenesis and enzyme kinetics","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p8223565] Probing the role of metal ions in the mechanism of inositol monophosphatase by site-directed mutagenesis. (1993). https://pubmed.ncbi.nlm.nih.gov/8223565/ DOI: 10.1111/j.1432-1033.1993.tb18244.x","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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