{"id":"adda80fc-8da8-5628-98e5-27133a6e6158","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-heart-acp-lipoylation-spared","predicate":"deletion_does_not_significantly_change","statement":"In the cardiac Ndufab1-knockout study, the measured mitochondrial protein lipoylation was not significantly changed despite respiratory defects.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"b419faaa-4905-5eb4-9916-17255b318761","mechanism_event_label":"Mouse heart respiratory failure occurred without the measured lipoylation defect seen in the cultured-cell study.","subject":{"id":"2b43af69-e964-5a70-896a-3f424d79d2d9","slug":"mouse-ndufab1","display_name":"Mouse mitochondrial acyl carrier protein / Ndufab1","entity_type_key":"protein"},"object":{"id":"a545cda4-692b-5513-a8f3-71c0afb0736c","slug":"mitochondrial-protein-lipoylation","display_name":"Mitochondrial protein lipoylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b419faaa-4905-5eb4-9916-17255b318761","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-heart-acp-lipoylation-spared-event","event_type":"biochemical_relationship","label":"Mouse heart respiratory failure occurred without the measured lipoylation defect seen in the cultured-cell study.","description":"In the cardiac Ndufab1-knockout study, the measured mitochondrial protein lipoylation was not significantly changed despite respiratory defects.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fd2197c3-5a63-55f4-8bb1-bfc1e91a0e1e","slug":"protein-bound-lipoamide","display_name":"Protein-bound oxidized lipoyl-lysine","entity_type_key":"chemical_species"},"role":"measured_cofactor_state","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"role":"related_cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b43af69-e964-5a70-896a-3f424d79d2d9","slug":"mouse-ndufab1","display_name":"Mouse mitochondrial acyl carrier protein / Ndufab1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a545cda4-692b-5513-a8f3-71c0afb0736c","slug":"mitochondrial-protein-lipoylation","display_name":"Mitochondrial protein lipoylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text line 47; Supplementary Fig. S4B","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cardiomyocyte-specific Ndufab1 knockout mice and isolated heart mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cardiomyocyte-specific Ndufab1 deletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A null result for these measurements is not proof that all fatty-acid synthesis was intact. Different tissue, developmental timing and depletion conditions preclude a same-context contradiction with HEK293T cells. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Mouse heart respiratory failure occurred without the measured lipoylation defect seen in the cultured-cell study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-ndufab2019] NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. (2019). https://pubmed.ncbi.nlm.nih.gov/31366990/ DOI: 10.1038/s41422-019-0208-x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Heart mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"4b70714f-186f-541f-bfe3-19f79f33311e","evidence_kind":"source_excerpt","locator":"Lines 977-989","start_line":977,"end_line":989,"excerpt":"### b5-met-heart-acp-lipoylation-spared\nIn the cardiac Ndufab1-knockout study, the measured mitochondrial protein lipoylation was not significantly changed despite respiratory defects.\nCondition category: machinery_impairment\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Mouse heart respiratory failure occurred without the measured lipoylation defect seen in the cultured-cell study.\norganism: Mus musculus\ntissue_or_cell_type: Heart mitochondria\nexperimental_model: Cardiomyocyte-specific Ndufab1 knockout mice and isolated heart mitochondria\nlimitations: A null result for these measurements is not proof that all fatty-acid synthesis was intact. Different tissue, developmental timing and depletion conditions preclude a same-context contradiction with HEK293T cells. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: Cardiomyocyte-specific Ndufab1 deletion.\ncross_nutrient: true\nevidence_location: Full text line 47; Supplementary Fig. S4B\n[b5-met-ndufab2019] NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. (2019). https://pubmed.ncbi.nlm.nih.gov/31366990/ DOI: 10.1038/s41422-019-0208-x","model_system":"Cardiomyocyte-specific Ndufab1 knockout mice and isolated heart mitochondria","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-ndufab2019] NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. (2019). https://pubmed.ncbi.nlm.nih.gov/31366990/ DOI: 10.1038/s41422-019-0208-x","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}