{"id":"adbda5a8-2503-562c-8365-6b64d2572f16","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-t47d-induction-null","predicate":"did_not_significantly_induce_in_t47d","statement":"DIM alone did not significantly induce CYP1A1 mRNA or EROD activity at concentrations up to 31 micromolar in this T47D system.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"580e4c9e-ce4e-5a08-989d-15dd1db623fe","mechanism_event_label":"The induction result is not universal across cell types and exposures.","subject":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"object":{"id":"95f0cce2-8f07-53f1-8cb7-3b5fb4338190","slug":"cyp1a1","display_name":"Human cytochrome P450 1A1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"580e4c9e-ce4e-5a08-989d-15dd1db623fe","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-t47d-induction-null-event","event_type":"biochemical_relationship","label":"The induction result is not universal across cell types and exposures.","description":"DIM alone did not significantly induce CYP1A1 mRNA or EROD activity at concentrations up to 31 micromolar in this T47D system.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"95f0cce2-8f07-53f1-8cb7-3b5fb4338190","slug":"cyp1a1","display_name":"Human cytochrome P450 1A1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/dim-research/8866829.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b6df68a4c8fabdb1ec4bf4c05e25c0ac350fe1505222b1423cd2124e26c9eb7f\", \"start_char\": 0, \"end_char\": 1403, \"text_sha256\": \"b6df68a4c8fabdb1ec4bf4c05e25c0ac350fe1505222b1423cd2124e26c9eb7f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Receptor competition, reporter and enzyme assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"DIM alone up to 31 micromolar or with TCDD","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Partial antagonism of a coadministered strong ligand is not absence of all AhR binding or a contradiction of induction in another cell type.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human T47D breast-cancer cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The induction result is not universal across cell types and exposures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dim-p8866829] Indole-3-carbinol and diindolylmethane as aryl hydrocarbon (Ah) receptor agonists and antagonists in T47D human breast cancer cells. (1996). https://pubmed.ncbi.nlm.nih.gov/8866829/ DOI: 10.1016/0006-2952(96)00060-3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"AhR-mediated CYP1A1 response","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4624ab95-9e12-5958-971f-63e23344d2ab","evidence_kind":"source_excerpt","locator":"Lines 337-348","start_line":337,"end_line":348,"excerpt":"### dim-t47d-induction-null\nDIM alone did not significantly induce CYP1A1 mRNA or EROD activity at concentrations up to 31 micromolar in this T47D system.\nCondition category: normal\nnutrient_topic: Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The induction result is not universal across cell types and exposures.\norganism: Human T47D breast-cancer cells\ntissue_or_cell_type: AhR-mediated CYP1A1 response\nexperimental_model: Receptor competition, reporter and enzyme assays\nlimitations: Partial antagonism of a coadministered strong ligand is not absence of all AhR binding or a contradiction of induction in another cell type.\nexposure: DIM alone up to 31 micromolar or with TCDD\nevidence_span: {\"source_cache\": \"artifacts/dim-research/8866829.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b6df68a4c8fabdb1ec4bf4c05e25c0ac350fe1505222b1423cd2124e26c9eb7f\", \"start_char\": 0, \"end_char\": 1403, \"text_sha256\": \"b6df68a4c8fabdb1ec4bf4c05e25c0ac350fe1505222b1423cd2124e26c9eb7f\"}\n[dim-p8866829] Indole-3-carbinol and diindolylmethane as aryl hydrocarbon (Ah) receptor agonists and antagonists in T47D human breast cancer cells. 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