{"id":"ada8201a-9986-546e-9c82-da0fab62c31d","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-tungstate-transport","predicate":"inhibits_heterologous_transport_by","statement":"Tungstate inhibited HsMOT2-dependent molybdate uptake in yeast; 1 mM sulfate had little effect.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"857019e9-b7b3-5b4e-a25e-3165359ba15f","mechanism_event_label":"Tungstate competed in this transporter assay, while sulfate had little effect.","subject":{"id":"9b22b727-d7d9-5731-a221-4aa8c7adcb4e","slug":"tungstate","display_name":"Tungstate / WO4(2-)","entity_type_key":"ion"},"object":{"id":"49b228c5-a2e8-56a3-82a9-de12dd6f3731","slug":"mfsd5","display_name":"Human MFSD5 / HsMOT2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"857019e9-b7b3-5b4e-a25e-3165359ba15f","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-tungstate-transport-event","event_type":"biochemical_relationship","label":"Tungstate competed in this transporter assay, while sulfate had little effect.","description":"Tungstate inhibited HsMOT2-dependent molybdate uptake in yeast; 1 mM sulfate had little effect.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"18479412-e52f-5921-8b5a-ddacefcbe083","slug":"molybdate","display_name":"Molybdate / MoO4(2-)","entity_type_key":"ion"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3634af03-823b-584f-8e8e-e6ab6e3fbb60","slug":"sulfate","display_name":"Sulfate / SO4(2-)","entity_type_key":"ion"},"role":"comparison","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9b22b727-d7d9-5731-a221-4aa8c7adcb4e","slug":"tungstate","display_name":"Tungstate / WO4(2-)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"49b228c5-a2e8-56a3-82a9-de12dd6f3731","slug":"mfsd5","display_name":"Human MFSD5 / HsMOT2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/21464289.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"861076d0403540a9fe3ad941cd7c0807224cb9323da6d8179546e9983f782cc7\", \"start_char\": 18693, \"end_char\": 18917, \"text_sha256\": \"df769097827247aa8c9b82a5877b016a6977021d43424f2deed3aad684c51688\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HsMOT2 expressed in Saccharomyces cerevisiae, with separate algal experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Molybdate uptake assays and oxyanion comparisons","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The text and figure use different tungstate concentrations; no exact tungstate dose is assigned here. Yeast assay, not human dietary competition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human protein in yeast; Chlamydomonas experiments separately","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Tungstate competed in this transporter assay, while sulfate had little effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p21464289] Algae and humans share a molybdate transporter. (2011). https://pubmed.ncbi.nlm.nih.gov/21464289/ DOI: 10.1073/pnas.1100700108","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Heterologous membrane transport","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9176d738-9c01-5eaf-821d-0a1496d0d2ce","evidence_kind":"source_excerpt","locator":"Lines 209-220","start_line":209,"end_line":220,"excerpt":"### mo-tungstate-transport\nTungstate inhibited HsMOT2-dependent molybdate uptake in yeast; 1 mM sulfate had little effect.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Tungstate competed in this transporter assay, while sulfate had little effect.\norganism: Human protein in yeast; Chlamydomonas experiments separately\ntissue_or_cell_type: Heterologous membrane transport\nexperimental_model: Human HsMOT2 expressed in Saccharomyces cerevisiae, with separate algal experiments\nlimitations: The text and figure use different tungstate concentrations; no exact tungstate dose is assigned here. Yeast assay, not human dietary competition.\nexposure: Molybdate uptake assays and oxyanion comparisons\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/21464289.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"861076d0403540a9fe3ad941cd7c0807224cb9323da6d8179546e9983f782cc7\", \"start_char\": 18693, \"end_char\": 18917, \"text_sha256\": \"df769097827247aa8c9b82a5877b016a6977021d43424f2deed3aad684c51688\"}\n[mo-p21464289] Algae and humans share a molybdate transporter. (2011). https://pubmed.ncbi.nlm.nih.gov/21464289/ DOI: 10.1073/pnas.1100700108","model_system":"Human HsMOT2 expressed in Saccharomyces cerevisiae, with separate algal experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mo-p21464289] Algae and humans share a molybdate transporter. (2011). https://pubmed.ncbi.nlm.nih.gov/21464289/ DOI: 10.1073/pnas.1100700108","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1aa6da60-8284-5252-8dd9-ac2250d5ced5","stable_key":"import-dc8975b1-95ff-5d9b-be17-a1c04610cca7","title":"Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"143147eb9ac7fe750bdd33bb50d7773bc0cd49cc94faf219aebce3c3d760dd8f","revision_id":"90c2239d-290b-5e16-815a-f94d30569ebd","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}