{"id":"ad825573-2010-594a-a3ba-53af39a84055","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-cxcl12-addback","predicate":"reduces","statement":"Adding mouse CXCL12 back to plasma from fisetin-treated old mice reduced dilation in recipient isolated mouse arteries.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"212eeb9d-abe6-530b-9d4d-1c90796e5782","mechanism_event_label":"Replacing one secreted factor weakened the vascular improvement.","subject":{"id":"563d0083-0065-5949-a288-9c8283864e0c","slug":"mouse-cxcl12","display_name":"Mouse chemokine CXCL12","entity_type_key":"cytokine"},"object":{"id":"2abaca69-1d23-54db-80b8-4bdb72804a83","slug":"mouse-arterial-endothelium-dependent-dilation","display_name":"Mouse artery endothelium-dependent dilation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"212eeb9d-abe6-530b-9d4d-1c90796e5782","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-cxcl12-addback-event","event_type":"observed_relationship","label":"Replacing one secreted factor weakened the vascular improvement.","description":"Adding mouse CXCL12 back to plasma from fisetin-treated old mice reduced dilation in recipient isolated mouse arteries.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"563d0083-0065-5949-a288-9c8283864e0c","slug":"mouse-cxcl12","display_name":"Mouse chemokine CXCL12","entity_type_key":"cytokine"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2abaca69-1d23-54db-80b8-4bdb72804a83","slug":"mouse-arterial-endothelium-dependent-dilation","display_name":"Mouse artery endothelium-dependent dilation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant CXCL12 matched old-control plasma concentration of 3210 pg/mL.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Partial mediation; other plasma components remain involved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Replacing one secreted factor weakened the vascular improvement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Senolytic Treatment With Fisetin Reverses Age-Related Endothelial Dysfunction Partially Mediated by SASP Factor CXCL12. · 2026 · https://pubmed.ncbi.nlm.nih.gov/42021544/ · DOI 10.1111/acel.70500","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d798539a-6d8b-593c-a8f4-d698ced09b30","evidence_kind":"source_excerpt","locator":"Lines 256-262","start_line":256,"end_line":262,"excerpt":"## fisetin-cxcl12-addback\nReplacing one secreted factor weakened the vascular improvement.\nAdding mouse CXCL12 back to plasma from fisetin-treated old mice reduced dilation in recipient isolated mouse arteries.\nModel: Recombinant CXCL12 matched old-control plasma concentration of 3210 pg/mL.\nLimitations: Partial mediation; other plasma components remain involved.\nEvidence access: Primary full text\nSenolytic Treatment With Fisetin Reverses Age-Related Endothelial Dysfunction Partially Mediated by SASP Factor CXCL12. · 2026 · https://pubmed.ncbi.nlm.nih.gov/42021544/ · DOI 10.1111/acel.70500","model_system":"Recombinant CXCL12 matched old-control plasma concentration of 3210 pg/mL.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}