{"id":"ad73c581-cbcd-5d8f-b9ea-554ffd91b806","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-trpm7-physiologic","predicate":"mediates","statement":"PLC-coupled agonists activated TRPM7 currents under perforated-patch conditions that preserved physiological intracellular ions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ab0add07-3f7c-57a1-8c90-f412cdd4afcd","mechanism_event_label":"The channel response reversed when the cell’s internal environment was better preserved.","subject":{"id":"30400645-395b-5719-b395-76d05db006d0","slug":"mouse-trpm7","display_name":"Mouse Trpm7 channel","entity_type_key":"protein"},"object":{"id":"4bb887d6-22d6-54e8-aff5-03e7d0ca2306","slug":"trpm7-current","display_name":"TRPM7-mediated membrane current","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ab0add07-3f7c-57a1-8c90-f412cdd4afcd","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-trpm7-physiologic-event","event_type":"biochemical_relationship","label":"The channel response reversed when the cell’s internal environment was better preserved.","description":"PLC-coupled agonists activated TRPM7 currents under perforated-patch conditions that preserved physiological intracellular ions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"30400645-395b-5719-b395-76d05db006d0","slug":"mouse-trpm7","display_name":"Mouse Trpm7 channel","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4bb887d6-22d6-54e8-aff5-03e7d0ca2306","slug":"trpm7-current","display_name":"TRPM7-mediated membrane current","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/17095511.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"28346917b1f99a6c832092a0ff4d39cb2906a680e5e18257412a19ba289d6c83\", \"start_char\": 0, \"end_char\": 1403, \"text_sha256\": \"28346917b1f99a6c832092a0ff4d39cb2906a680e5e18257412a19ba289d6c83\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Perforated versus whole-cell patch clamp and FRET","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"PLC-coupled agonists with different intracellular magnesium conditions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The opposite current responses are explicitly explained by experimental ionic conditions; they are not an unresolved scientific contradiction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mouse N1E-115 neuroblastoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The channel response reversed when the cell’s internal environment was better preserved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p17095511] Activation of TRPM7 channels by phospholipase C-coupled receptor agonists. (2007). https://pubmed.ncbi.nlm.nih.gov/17095511/ DOI: 10.1074/jbc.m605300200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"TRPM7-expressing cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"20ccdf88-86ad-59aa-be22-98ff9ce86355","evidence_kind":"source_excerpt","locator":"Lines 977-988","start_line":977,"end_line":988,"excerpt":"### ino-trpm7-physiologic\nPLC-coupled agonists activated TRPM7 currents under perforated-patch conditions that preserved physiological intracellular ions.\nCondition category: normal\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The channel response reversed when the cell’s internal environment was better preserved.\norganism: Mouse N1E-115 neuroblastoma cells\ntissue_or_cell_type: TRPM7-expressing cells\nexperimental_model: Perforated versus whole-cell patch clamp and FRET\nlimitations: The opposite current responses are explicitly explained by experimental ionic conditions; they are not an unresolved scientific contradiction.\nexposure: PLC-coupled agonists with different intracellular magnesium conditions\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/17095511.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"28346917b1f99a6c832092a0ff4d39cb2906a680e5e18257412a19ba289d6c83\", \"start_char\": 0, \"end_char\": 1403, \"text_sha256\": \"28346917b1f99a6c832092a0ff4d39cb2906a680e5e18257412a19ba289d6c83\"}\n[ino-p17095511] Activation of TRPM7 channels by phospholipase C-coupled receptor agonists. (2007). https://pubmed.ncbi.nlm.nih.gov/17095511/ DOI: 10.1074/jbc.m605300200","model_system":"Perforated versus whole-cell patch clamp and FRET","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p17095511] Activation of TRPM7 channels by phospholipase C-coupled receptor agonists. (2007). https://pubmed.ncbi.nlm.nih.gov/17095511/ DOI: 10.1074/jbc.m605300200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f7adbcedad647d5f8a3cc9e46bfa515e83c508be9c2a4e9d59c5f5dfb0667d53","revision_id":"a82d187c-60fb-52d1-8482-eadb92860f33","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}