{"id":"ad63458d-c995-56ed-b5bb-b27e6948d15e","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-pump-mg-mn","predicate":"increases_vanadate_affinity","statement":"Replacing 3 mM MgCl2 with MnCl2 lowered vanadate-binding Kd from 96 to 12 nM in the dog-kidney Na/K-ATPase preparation without changing binding capacity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"07ac4a2e-0e51-5f6d-8567-9bf035f17378","mechanism_event_label":"The supporting divalent ion changed inhibitor affinity.","subject":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"object":{"id":"4d1e42e4-30e4-53aa-b967-9a8e2ed3043a","slug":"dog-kidney-na-k-atpase","display_name":"Dog kidney sodium/potassium ATPase preparation","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"07ac4a2e-0e51-5f6d-8567-9bf035f17378","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-pump-mg-mn-event","event_type":"observed_relationship","label":"The supporting divalent ion changed inhibitor affinity.","description":"Replacing 3 mM MgCl2 with MnCl2 lowered vanadate-binding Kd from 96 to 12 nM in the dog-kidney Na/K-ATPase preparation without changing binding capacity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4d1e42e4-30e4-53aa-b967-9a8e2ed3043a","slug":"dog-kidney-na-k-atpase","display_name":"Dog kidney sodium/potassium ATPase preparation","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Dog kidney particulate-enzyme radiovanadate binding assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay concentrations and enzyme state matter; not a manganese supplementation interaction in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"The supporting divalent ion changed inhibitor affinity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Vanadate binding to the (Na + K)-ATPase. · 1981 · https://pubmed.ncbi.nlm.nih.gov/6277881/ · DOI 10.1007/BF00743200","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"704e9b9e-5b93-596d-9ad2-b29ce0b9ee0f","evidence_kind":"source_excerpt","locator":"Lines 230-236","start_line":230,"end_line":236,"excerpt":"## vanadium-pump-mg-mn\nThe supporting divalent ion changed inhibitor affinity.\nReplacing 3 mM MgCl2 with MnCl2 lowered vanadate-binding Kd from 96 to 12 nM in the dog-kidney Na/K-ATPase preparation without changing binding capacity.\nModel: Dog kidney particulate-enzyme radiovanadate binding assay.\nLimitations: Assay concentrations and enzyme state matter; not a manganese supplementation interaction in humans.\nEvidence access: Primary abstract\nVanadate binding to the (Na + K)-ATPase. · 1981 · https://pubmed.ncbi.nlm.nih.gov/6277881/ · DOI 10.1007/BF00743200","model_system":"Dog kidney particulate-enzyme radiovanadate binding assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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