{"id":"ad293fbe-c746-5ebc-91c9-a1a883cf1318","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-tf-crystal-transformation","predicate":"forms_after_reaction","statement":"Crystals of C-lobe-ferric human transferrin exposed to vanadyl acetylacetonate contained a two-vanadium(V) unit linked to Tyr188 rather than intact V(IV) acetylacetonate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"16412eb3-2090-52f8-b1fb-388890641d3f","mechanism_event_label":"The species bound to a protein can differ from the compound originally added.","subject":{"id":"664d6382-87fc-577a-9a28-ae713035d2b1","slug":"vanadyl-acetylacetonate","display_name":"Bis(acetylacetonato)oxovanadium(IV)","entity_type_key":"chemical_species"},"object":{"id":"1b146e35-4788-54cf-b511-185b4b6736bc","slug":"human-transferrin-tyr188-divanadate-adduct","display_name":"Human C-lobe-ferric transferrin Tyr188-linked divanadate(V) adduct","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"16412eb3-2090-52f8-b1fb-388890641d3f","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-tf-crystal-transformation-event","event_type":"observed_relationship","label":"The species bound to a protein can differ from the compound originally added.","description":"Crystals of C-lobe-ferric human transferrin exposed to vanadyl acetylacetonate contained a two-vanadium(V) unit linked to Tyr188 rather than intact V(IV) acetylacetonate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"664d6382-87fc-577a-9a28-ae713035d2b1","slug":"vanadyl-acetylacetonate","display_name":"Bis(acetylacetonato)oxovanadium(IV)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1b146e35-4788-54cf-b511-185b4b6736bc","slug":"human-transferrin-tyr188-divanadate-adduct","display_name":"Human C-lobe-ferric transferrin Tyr188-linked divanadate(V) adduct","entity_type_key":"protein_state"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ac656bc2-967c-568b-b6d5-58c7ff433c94","slug":"tf","display_name":"Human serum transferrin / TF","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"964be7ac-43bd-5f3e-8326-94f0590de67a","slug":"iron-iii","display_name":"Ferric iron","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human protein crystallography; 5 mM compound and crystallization conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reaction/crystal structure does not demonstrate the same species dominates human blood.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"The species bound to a protein can differ from the compound originally added.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"First crystal structure of an adduct formed upon reaction of a vanadium compound with human serum transferrin. · 2026 · https://pubmed.ncbi.nlm.nih.gov/41545537/ · DOI 10.1038/s42004-026-01891-1","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0c810a99-3c45-5f62-8dc0-2f836e0b8ac1","evidence_kind":"source_excerpt","locator":"Lines 214-220","start_line":214,"end_line":220,"excerpt":"## vanadium-tf-crystal-transformation\nThe species bound to a protein can differ from the compound originally added.\nCrystals of C-lobe-ferric human transferrin exposed to vanadyl acetylacetonate contained a two-vanadium(V) unit linked to Tyr188 rather than intact V(IV) acetylacetonate.\nModel: Human protein crystallography; 5 mM compound and crystallization conditions.\nLimitations: Reaction/crystal structure does not demonstrate the same species dominates human blood.\nEvidence access: Primary full text\nFirst crystal structure of an adduct formed upon reaction of a vanadium compound with human serum transferrin. · 2026 · https://pubmed.ncbi.nlm.nih.gov/41545537/ · DOI 10.1038/s42004-026-01891-1","model_system":"Human protein crystallography; 5 mM compound and crystallization conditions.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"d36b30d972e756ca4d19b66f976980e206c65025bd4bbf4c127c17320bd019c2","revision_id":"70d42967-8032-5627-8670-f59841735e89","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}