{"id":"ad192eaa-a8a8-5336-90b6-fc460aa389b1","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:trpv5-direct-calcium-permeation","predicate":"transports","statement":"Cloned rabbit ECaC, now termed TRPV5, confers calcium influx when expressed in Xenopus oocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bea89716-8a3b-5229-a9a9-38c83fa01711","mechanism_event_label":"TRPV5 provides a calcium entry channel.","subject":{"id":"ae79ed63-956e-5f19-b2bc-0142bb6e9aea","slug":"trpv5","display_name":"TRPV5","entity_type_key":"protein"},"object":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"bea89716-8a3b-5229-a9a9-38c83fa01711","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:trpv5-direct-calcium-permeation-event","event_type":"biochemical_relationship","label":"TRPV5 provides a calcium entry channel.","description":"Cloned rabbit ECaC, now termed TRPV5, confers calcium influx when expressed in Xenopus oocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ae79ed63-956e-5f19-b2bc-0142bb6e9aea","slug":"trpv5","display_name":"TRPV5","entity_type_key":"protein"},"role":"channel","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"transported_ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Rabbit ECaC/TRPV5 cloning, epithelial localization and expression in Xenopus oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression assay; epithelial localization supports an apical role but does not quantify all renal calcium transport.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Oryctolagus cuniculus channel in Xenopus laevis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"TRPV5 provides a calcium entry channel.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hoenderop1999] Molecular identification of the apical Ca2+ channel in 1,25-dihydroxyvitamin D3-responsive epithelia (1999). https://pubmed.ncbi.nlm.nih.gov/10085067/ DOI: 10.1074/jbc.274.13.8375","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Plasma membrane; renal epithelial channel origin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Confers calcium influx when expressed.","entity":null},{"dimension":"transport_or_reaction_direction","value_text":"inward","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_pool","value_text":"cytosolic calcium","comparator":null,"unit":null,"notes":"Confers calcium influx when expressed.","entity":null}],"evidence":[{"id":"950289de-48ae-5d73-9e0c-aeafad179618","evidence_kind":"source_excerpt","locator":"Lines 375-385","start_line":375,"end_line":385,"excerpt":"### trpv5-direct-calcium-permeation\nCloned rabbit ECaC, now termed TRPV5, confers calcium influx when expressed in Xenopus oocytes.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: TRPV5 provides a calcium entry channel.\norganism: Oryctolagus cuniculus channel in Xenopus laevis\ntissue_or_cell_type: Plasma membrane; renal epithelial channel origin\nexperimental_model: Rabbit ECaC/TRPV5 cloning, epithelial localization and expression in Xenopus oocytes\nlimitations: Expression assay; epithelial localization supports an apical role but does not quantify all renal calcium transport.\ntransport_or_reaction_direction: inward\n[hoenderop1999] Molecular identification of the apical Ca2+ channel in 1,25-dihydroxyvitamin D3-responsive epithelia (1999). https://pubmed.ncbi.nlm.nih.gov/10085067/ DOI: 10.1074/jbc.274.13.8375","model_system":"Rabbit ECaC/TRPV5 cloning, epithelial localization and expression in Xenopus oocytes","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [hoenderop1999] Molecular identification of the apical Ca2+ channel in 1,25-dihydroxyvitamin D3-responsive epithelia (1999). https://pubmed.ncbi.nlm.nih.gov/10085067/ DOI: 10.1074/jbc.274.13.8375","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}