{"id":"acb1693c-419b-569a-9f63-3ef2953fb57a","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-enz-mouse-low-mn-iron-sod2","predicate":"increases_under_restriction","statement":"Mice fed the lower-manganese diet for four weeks had approximately 30% of isolated liver Sod2 iron-loaded, versus negligible iron loading in controls.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"f60ea369-0775-5d61-8526-cc138953dab7","mechanism_event_label":"Lower dietary manganese relative to iron changed liver Sod2 metal loading in mice.","subject":{"id":"f0459455-8231-513b-8214-7ea5b3259b4b","slug":"manganese","display_name":"Manganese","entity_type_key":"nutrient_element"},"object":{"id":"cd79f740-d578-5fb0-b3da-1fc30bc74a23","slug":"mouse-sod2-iron-loaded","display_name":"Iron-loaded mouse Sod2","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"f60ea369-0775-5d61-8526-cc138953dab7","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-enz-mouse-low-mn-iron-sod2-event","event_type":"biochemical_relationship","label":"Lower dietary manganese relative to iron changed liver Sod2 metal loading in mice.","description":"Mice fed the lower-manganese diet for four weeks had approximately 30% of isolated liver Sod2 iron-loaded, versus negligible iron loading in controls.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f0459455-8231-513b-8214-7ea5b3259b4b","slug":"manganese","display_name":"Manganese","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cd79f740-d578-5fb0-b3da-1fc30bc74a23","slug":"mouse-sod2-iron-loaded","display_name":"Iron-loaded mouse Sod2","entity_type_key":"protein_state"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"competing metal identity","stoichiometry":null,"state_label":"","sequence_order":2,"notes":"Element identity only; no fixed oxidation state assigned to total tissue iron or iron throughout the enzyme redox reaction."}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Dietary Mn:Fe balance affected mouse Sod2 metal occupancy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified SOD2, SOD2-overexpressing human cells, and dietary C57BL/6J mouse experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Four weeks of 40 ppm Mn/275 ppm Fe versus 150 ppm Mn/275 ppm Fe control.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Primary experimental scope only; no human dietary threshold, clinical treatment rule, or universal metal substitution is established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Manganese research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"manganese","display_name":"Manganese","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Lower dietary manganese relative to iron changed liver Sod2 metal loading in mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mn-enz-29398562] Switch of Mitochondrial Superoxide Dismutase into a Prooxidant Peroxidase in Manganese-Deficient Cells and Mice. (2018). https://pubmed.ncbi.nlm.nih.gov/29398562/ DOI: 10.1016/j.chembiol.2018.01.007","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver Sod2","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"be013a20-c122-571b-b6a2-05a604140c01","evidence_kind":"source_excerpt","locator":"Lines 508-519","start_line":508,"end_line":519,"excerpt":"### mn-enz-mouse-low-mn-iron-sod2\nMice fed the lower-manganese diet for four weeks had approximately 30% of isolated liver Sod2 iron-loaded, versus negligible iron loading in controls.\nCondition category: nutrient_deficiency\nnutrient_topic: Manganese research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Lower dietary manganese relative to iron changed liver Sod2 metal loading in mice.\norganism: Mus musculus\ntissue_or_cell_type: Liver Sod2\nexperimental_model: Purified SOD2, SOD2-overexpressing human cells, and dietary C57BL/6J mouse experiments\nlimitations: Primary experimental scope only; no human dietary threshold, clinical treatment rule, or universal metal substitution is established.\nexposure: Four weeks of 40 ppm Mn/275 ppm Fe versus 150 ppm Mn/275 ppm Fe control.\ncross_nutrient: Dietary Mn:Fe balance affected mouse Sod2 metal occupancy.\n[mn-enz-29398562] Switch of Mitochondrial Superoxide Dismutase into a Prooxidant Peroxidase in Manganese-Deficient Cells and Mice. 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