{"id":"abf47bc4-0073-51b6-9157-9cf5faa782df","stable_key":"d8050016-13c9-5391-9d61-63de73590053:zeaxanthin-ascorbate-rescue-limit","predicate":"fails_to_prevent","statement":"The vitamin E–zeaxanthin combination did not prevent the deleterious high-ascorbate effect in the rose-bengal model.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"12b751a7-d0d3-5fc8-8840-0f8b1b0465dd","mechanism_event_label":"Protection had limits under a different light challenge.","subject":{"id":"9f07b6db-bca0-5e51-932c-d1dcc57e7f5f","slug":"zeaxanthin-alpha-tocopherol-assay-mixture","display_name":"Zeaxanthin and alpha-tocopherol assay combination","entity_type_key":"chemical_species"},"object":{"id":"051f0916-0dd7-59b9-8037-c565bdf63bd6","slug":"arpe19-rose-bengal-phototoxicity","display_name":"ARPE-19 rose-bengal photosensitization toxicity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"12b751a7-d0d3-5fc8-8840-0f8b1b0465dd","stable_key":"d8050016-13c9-5391-9d61-63de73590053:zeaxanthin-ascorbate-rescue-limit-event","event_type":"biochemical_relationship","label":"Protection had limits under a different light challenge.","description":"The vitamin E–zeaxanthin combination did not prevent the deleterious high-ascorbate effect in the rose-bengal model.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0a60c685-7502-5ece-9da2-2c1a2e3bcdf1","slug":"zeaxanthin","display_name":"Dietary (3R,3-prime-R)-zeaxanthin","entity_type_key":"small_molecule"},"role":"tested_antioxidant","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"role":"tested_antioxidant","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"concentration_dependent_component","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9f07b6db-bca0-5e51-932c-d1dcc57e7f5f","slug":"zeaxanthin-alpha-tocopherol-assay-mixture","display_name":"Zeaxanthin and alpha-tocopherol assay combination","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"051f0916-0dd7-59b9-8037-c565bdf63bd6","slug":"arpe19-rose-bengal-phototoxicity","display_name":"ARPE-19 rose-bengal photosensitization toxicity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/zeaxanthin-research/22924673.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0acdf0e77d224421d61b41baeefd396ff54e53488a85dee22bfb04b0f827c177\", \"start_char\": 0, \"end_char\": 1415, \"text_sha256\": \"0acdf0e77d224421d61b41baeefd396ff54e53488a85dee22bfb04b0f827c177\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rose-bengal photosensitization with antioxidant combinations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Visible-light exposures; ascorbate 0.35–1.4 mM with lipophilic antioxidants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different photosensitizer and exposure duration from the retinaldehyde study; do not treat this as a universal contradiction or dietary safety threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zeaxanthin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zeaxanthin","display_name":"Dietary (3R,3-prime-R)-zeaxanthin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human ARPE-19 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Protection had limits under a different light challenge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zeaxanthin-p22924673] Concentration dependence of vitamin C in combinations with vitamin E and zeaxanthin on light-induced toxicity to retinal pigment epithelial cells. (2012). https://pubmed.ncbi.nlm.nih.gov/22924673/ DOI: 10.1111/j.1751-1097.2012.01228.x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cultured RPE model","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4433241c-9a97-5a76-96df-dce7cf670a60","evidence_kind":"source_excerpt","locator":"Lines 964-975","start_line":964,"end_line":975,"excerpt":"### zeaxanthin-ascorbate-rescue-limit\nThe vitamin E–zeaxanthin combination did not prevent the deleterious high-ascorbate effect in the rose-bengal model.\nCondition category: normal\nnutrient_topic: Zeaxanthin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Protection had limits under a different light challenge.\norganism: Human ARPE-19 cells\ntissue_or_cell_type: Cultured RPE model\nexperimental_model: Rose-bengal photosensitization with antioxidant combinations\nlimitations: Different photosensitizer and exposure duration from the retinaldehyde study; do not treat this as a universal contradiction or dietary safety threshold.\nexposure: Visible-light exposures; ascorbate 0.35–1.4 mM with lipophilic antioxidants\nevidence_span: {\"source_cache\": \"artifacts/zeaxanthin-research/22924673.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0acdf0e77d224421d61b41baeefd396ff54e53488a85dee22bfb04b0f827c177\", \"start_char\": 0, \"end_char\": 1415, \"text_sha256\": \"0acdf0e77d224421d61b41baeefd396ff54e53488a85dee22bfb04b0f827c177\"}\n[zeaxanthin-p22924673] Concentration dependence of vitamin C in combinations with vitamin E and zeaxanthin on light-induced toxicity to retinal pigment epithelial cells. (2012). https://pubmed.ncbi.nlm.nih.gov/22924673/ DOI: 10.1111/j.1751-1097.2012.01228.x","model_system":"Rose-bengal photosensitization with antioxidant combinations","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zeaxanthin-p22924673] Concentration dependence of vitamin C in combinations with vitamin E and zeaxanthin on light-induced toxicity to retinal pigment epithelial cells. (2012). https://pubmed.ncbi.nlm.nih.gov/22924673/ DOI: 10.1111/j.1751-1097.2012.01228.x","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"df7effe5-d60c-525e-bd48-1518e1a03c51","stable_key":"import-d8050016-13c9-5391-9d61-63de73590053","title":"Zeaxanthin: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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