{"id":"abe65595-ebdd-5866-8612-5c9b14af5050","stable_key":"cb41aa31-e26b-5b60-b7e0-e4985fb7aea8:hydroxytyrosol-tyrosol-cyp-conversion","predicate":"is_hydroxylated_to","statement":"Human liver microsomes and recombinant enzymes converted tyrosol to hydroxytyrosol, with CYP2A6 and CYP2D6 supported as major contributors and CYP3A4 also active in baculosomes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"90e331d4-3c9c-5c26-9152-c3ec0fcd461c","mechanism_event_label":"Human liver microsomes and recombinant enzymes converted tyrosol to hydroxytyrosol, with CYP2A6 and CYP2D6 supported as major contributors and CYP3A4 also active in baculosomes.","subject":{"id":"677d24bc-5068-58d2-a9fb-91610c5f1983","slug":"tyrosol","display_name":"Tyrosol","entity_type_key":"small_molecule"},"object":{"id":"45a7aaec-8acc-5d5a-a014-c483ef5385e2","slug":"hydroxytyrosol","display_name":"Hydroxytyrosol","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"90e331d4-3c9c-5c26-9152-c3ec0fcd461c","stable_key":"cb41aa31-e26b-5b60-b7e0-e4985fb7aea8:hydroxytyrosol-tyrosol-cyp-conversion-event","event_type":"observed_relationship","label":"Human liver microsomes and recombinant enzymes converted tyrosol to hydroxytyrosol, with CYP2A6 and CYP2D6 supported as major contributors and CYP3A4 also active in baculosomes.","description":"Human liver microsomes and recombinant enzymes converted tyrosol to hydroxytyrosol, with CYP2A6 and CYP2D6 supported as major contributors and CYP3A4 also active in baculosomes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"677d24bc-5068-58d2-a9fb-91610c5f1983","slug":"tyrosol","display_name":"Tyrosol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"45a7aaec-8acc-5d5a-a014-c483ef5385e2","slug":"hydroxytyrosol","display_name":"Hydroxytyrosol","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"44b7b150-3615-5d52-9db8-beb212fc31eb","slug":"cyp2a6","display_name":"Human cytochrome P450 2A6 / CYP2A6","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"10f7a103-d63d-5bf4-8384-c5e1308ce126","slug":"cyp2d6","display_name":"Human cytochrome P450 2D6","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"54e95ff1-4ffa-5e6f-a4cb-b47e5d2a5bda","slug":"cyp3a4","display_name":"Human cytochrome P450 3A4","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Tyrosol with selective CYP inhibitors or recombinant enzymes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Enzyme incubation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human liver microsomes, recombinant CYPs, genotyped livers and rat control experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Relative contribution varies by genotype and setting; this does not make every tyrosol food equivalent to hydroxytyrosol dosing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Hydroxytyrosol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"hydroxytyrosol","display_name":"Hydroxytyrosol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human liver microsomes, recombinant CYPs, genotyped livers and rat control experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Human liver microsomes and recombinant enzymes converted tyrosol to hydroxytyrosol, with CYP2A6 and CYP2D6 supported as major contributors and CYP3A4 also active in baculosomes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"CYP2D6 and CYP2A6 biotransform dietary tyrosol into hydroxytyrosol. (2017). https://pubmed.ncbi.nlm.nih.gov/27664690/ DOI: 10.1016/j.foodchem.2016.09.026","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro and in vivo experimental metabolism","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Tyrosol hydroxylation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"94f3e0cd-b371-546b-afab-15d3510d9ea6","evidence_kind":"source_excerpt","locator":"Lines 44-53","start_line":44,"end_line":53,"excerpt":"## hydroxytyrosol-tyrosol-cyp-conversion\nHuman liver microsomes and recombinant enzymes converted tyrosol to hydroxytyrosol, with CYP2A6 and CYP2D6 supported as major contributors and CYP3A4 also active in baculosomes.\nModel/species: Human liver microsomes, recombinant CYPs, genotyped livers and rat control experiments\nTissue/system: Tyrosol hydroxylation\nExposure: Tyrosol with selective CYP inhibitors or recombinant enzymes\nRoute: In vitro and in vivo experimental metabolism\nDuration: Enzyme incubation\nLimits: Relative contribution varies by genotype and setting; this does not make every tyrosol food equivalent to hydroxytyrosol dosing.\nPrimary reference: CYP2D6 and CYP2A6 biotransform dietary tyrosol into hydroxytyrosol. (2017). https://pubmed.ncbi.nlm.nih.gov/27664690/ DOI: 10.1016/j.foodchem.2016.09.026\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Human liver microsomes, recombinant CYPs, genotyped livers and rat control experiments","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f83cd982-34f0-55d4-8563-eedfb0f009f2","stable_key":"import-cb41aa31-e26b-5b60-b7e0-e4985fb7aea8","title":"Hydroxytyrosol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"7677fedffc16af238fc1ace3f88d206dd42b29b163c40b6e69ba6ed2457a3aee","revision_id":"1e0bd617-6834-5a2d-a74e-c058967d99b7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}