{"id":"abd6cd60-3c1e-579a-a914-c18a435b03df","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-comt-substrate","predicate":"reported_relationship","statement":"Porcine COMT with SAM generated a methylated RA standard used to identify methyl-RA.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"02c16242-c4c8-5f86-ab6b-b57f5679316a","mechanism_event_label":"Porcine COMT with SAM generated a methylated RA standard used to identify methyl-RA.","subject":{"id":"1bc88150-7233-5508-8319-746eac288b51","slug":"porcine-comt","display_name":"Porcine catechol O-methyltransferase","entity_type_key":"protein"},"object":{"id":"a00c5b15-3afd-5b6b-b817-a3f78f6ca3cd","slug":"methyl-rosmarinic-acid","display_name":"O-Methylrosmarinic acid, site unresolved","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"02c16242-c4c8-5f86-ab6b-b57f5679316a","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-comt-substrate-event","event_type":"biochemical_relationship","label":"Porcine COMT with SAM generated a methylated RA standard used to identify methyl-RA.","description":"### ra-comt-substrate\n\nPorcine COMT with SAM generated a methylated RA standard used to identify methyl-RA.\nDemonstrates enzymatic methylation, not an in-vivo rate or pool depletion.\nModel and exposure: Commercial porcine liver COMT and SAM; publisher-indexed Results.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Function of Plectranthus barbatus herbal tea as neuronal acetylcholinesterase inhibitor. | 2011 | https://pubmed.ncbi.nlm.nih.gov/21779558/ | https://doi.org/10.1039/c0fo00070a | Access: Indexed primary abstract plus publisher-indexed Results describing the porcine COMT/SAM methylated standard. Full Methods access unavailable.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1bc88150-7233-5508-8319-746eac288b51","slug":"porcine-comt","display_name":"Porcine catechol O-methyltransferase","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"methyl donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a00c5b15-3afd-5b6b-b817-a3f78f6ca3cd","slug":"methyl-rosmarinic-acid","display_name":"O-Methylrosmarinic acid, site unresolved","entity_type_key":"chemical_species"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e84829b1-0607-558e-8add-aaa3af4747e7","slug":"s-adenosylhomocysteine","display_name":"S-Adenosyl-L-homocysteine","entity_type_key":"small_molecule"},"role":"methyl-transfer coproduct","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract plus publisher-indexed Results describing the porcine COMT/SAM methylated standard. Full Methods access unavailable.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Commercial porcine liver COMT and SAM; publisher-indexed Results.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Porcine COMT with SAM generated a methylated RA standard used to identify methyl-RA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Function of Plectranthus barbatus herbal tea as neuronal acetylcholinesterase inhibitor. | https://pubmed.ncbi.nlm.nih.gov/21779558/ | https://doi.org/10.1039/c0fo00070a","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-comt-substrate; Demonstrates enzymatic methylation, not an in-vivo rate or pool depletion.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"37cf8030-1420-5e1c-bc53-afb42bc8a287","evidence_kind":"source_excerpt","locator":"Lines 105-111","start_line":105,"end_line":111,"excerpt":"### ra-comt-substrate\n\nPorcine COMT with SAM generated a methylated RA standard used to identify methyl-RA.\nDemonstrates enzymatic methylation, not an in-vivo rate or pool depletion.\nModel and exposure: Commercial porcine liver COMT and SAM; publisher-indexed Results.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Function of Plectranthus barbatus herbal tea as neuronal acetylcholinesterase inhibitor. | 2011 | https://pubmed.ncbi.nlm.nih.gov/21779558/ | https://doi.org/10.1039/c0fo00070a | Access: Indexed primary abstract plus publisher-indexed Results describing the porcine COMT/SAM methylated standard. Full Methods access unavailable.","model_system":"Commercial porcine liver COMT and SAM; publisher-indexed Results.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}