{"id":"abc5a7d7-ef19-5494-ae14-437e579f5754","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-epigenetic-clock-pilot","predicate":"associated_with","statement":"A 19-person DQF pilot reported nonsignificant increases in selected epigenetic-age measures after six months, unlike increases in an earlier DQ cohort.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1033926e-3315-529a-b467-8be92c56ab44","mechanism_event_label":"This pilot did not isolate a fisetin effect or show age reversal.","subject":{"id":"e68aa8b6-9ab4-5eac-9250-a28ef3df4778","slug":"dasatinib-quercetin-fisetin-combination","display_name":"Dasatinib-quercetin-fisetin combination","entity_type_key":"cellular_process"},"object":{"id":"4fe7384f-287a-521f-9e54-9b3fd231f774","slug":"human-dqf-epigenetic-clock-response","display_name":"Human DNA-methylation-clock response during DQF pilot","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1033926e-3315-529a-b467-8be92c56ab44","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-epigenetic-clock-pilot-event","event_type":"observed_relationship","label":"This pilot did not isolate a fisetin effect or show age reversal.","description":"A 19-person DQF pilot reported nonsignificant increases in selected epigenetic-age measures after six months, unlike increases in an earlier DQ cohort.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e68aa8b6-9ab4-5eac-9250-a28ef3df4778","slug":"dasatinib-quercetin-fisetin-combination","display_name":"Dasatinib-quercetin-fisetin combination","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4fe7384f-287a-521f-9e54-9b3fd231f774","slug":"human-dqf-epigenetic-clock-response","display_name":"Human DNA-methylation-clock response during DQF pilot","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Sequential cohorts, ten overlapping participants; combined drug/flavonoid exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a randomized fisetin-only comparison; changing immune-cell proportions can affect clock interpretation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"This pilot did not isolate a fisetin effect or show age reversal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Exploring the effects of Dasatinib, Quercetin, and Fisetin on DNA methylation clocks: a longitudinal study on senolytic interventions. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38393697/ · DOI 10.18632/aging.205581","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"6bf1cc0a-f99b-5e08-896d-f3ea258eaed9","evidence_kind":"source_excerpt","locator":"Lines 536-542","start_line":536,"end_line":542,"excerpt":"## fisetin-epigenetic-clock-pilot\nThis pilot did not isolate a fisetin effect or show age reversal.\nA 19-person DQF pilot reported nonsignificant increases in selected epigenetic-age measures after six months, unlike increases in an earlier DQ cohort.\nModel: Sequential cohorts, ten overlapping participants; combined drug/flavonoid exposure.\nLimitations: Not a randomized fisetin-only comparison; changing immune-cell proportions can affect clock interpretation.\nEvidence access: Primary abstract\nExploring the effects of Dasatinib, Quercetin, and Fisetin on DNA methylation clocks: a longitudinal study on senolytic interventions. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38393697/ · DOI 10.18632/aging.205581","model_system":"Sequential cohorts, ten overlapping participants; combined drug/flavonoid exposure.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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