{"id":"ab7488a4-0de3-5d0b-8321-ca057b86560f","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-microbial-route","predicate":"required_for_synthesis_of","statement":"Deleting the carboxypolyamine-decarboxylase orthologue disrupted spermidine synthesis in Campylobacter jejuni.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"71056a7f-2eec-5e98-a277-911cb2a6fdfb","mechanism_event_label":"Bacteria can use a route different from the human SAM pathway.","subject":{"id":"1402ca08-4b69-5878-92c8-143115e56fc9","slug":"campylobacter-jejuni-carboxyspermidine-decarboxylase","display_name":"Campylobacter jejuni carboxyspermidine decarboxylase","entity_type_key":"protein"},"object":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"71056a7f-2eec-5e98-a277-911cb2a6fdfb","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-microbial-route-event","event_type":"observed_relationship","label":"Bacteria can use a route different from the human SAM pathway.","description":"Deleting the carboxypolyamine-decarboxylase orthologue disrupted spermidine synthesis in Campylobacter jejuni.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1402ca08-4b69-5878-92c8-143115e56fc9","slug":"campylobacter-jejuni-carboxyspermidine-decarboxylase","display_name":"Campylobacter jejuni carboxyspermidine decarboxylase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b5293dc0-4c93-585f-bd51-50d3955cf226","slug":"carboxyspermidine","display_name":"Carboxyspermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Bacterial gene deletion and polyamine analysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The paper’s microbiome-wide prevalence claim is based on genomic inference; not measured production in each human.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Bacteria can use a route different from the human SAM pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Alternative spermidine biosynthetic route is critical for growth of Campylobacter jejuni and is the dominant polyamine pathway in human gut microbiota. · 2011 · https://pubmed.ncbi.nlm.nih.gov/22025614/ · DOI 10.1074/jbc.M111.307835","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"882ee50f-59ed-5401-9815-b99530dcf435","evidence_kind":"source_excerpt","locator":"Lines 414-420","start_line":414,"end_line":420,"excerpt":"## spermidine-microbial-route\nBacteria can use a route different from the human SAM pathway.\nDeleting the carboxypolyamine-decarboxylase orthologue disrupted spermidine synthesis in Campylobacter jejuni.\nModel: Bacterial gene deletion and polyamine analysis.\nLimitations: The paper’s microbiome-wide prevalence claim is based on genomic inference; not measured production in each human.\nEvidence access: Primary abstract\nAlternative spermidine biosynthetic route is critical for growth of Campylobacter jejuni and is the dominant polyamine pathway in human gut microbiota. · 2011 · https://pubmed.ncbi.nlm.nih.gov/22025614/ · DOI 10.1074/jbc.M111.307835","model_system":"Bacterial gene deletion and polyamine analysis.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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