{"id":"ab67f4a3-823b-5a44-b032-f6b4ea1e07d3","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-human-aldolase-boundary","predicate":"does_not_supply","statement":"Human GLY1 contained frameshifting deletions and a premature stop codon; its mRNA was not detected, unlike the transcribed mouse homolog.","claim_class":"identity","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"b6feb3b5-050b-588a-a19c-5308ca800c98","mechanism_event_label":"A second proposed glycine-producing route also differs across species.","subject":{"id":"3632f314-c57b-5b65-8b73-53c4438b8f3f","slug":"human-gly1-pseudogene","display_name":"Human threonine aldolase / GLY1 pseudogene","entity_type_key":"gene"},"object":{"id":"f2b2e71c-d8e4-559f-b24b-fdedb5e9cb80","slug":"human-functional-gly1-route","display_name":"Functional canonical human GLY1 threonine-aldolase route","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b6feb3b5-050b-588a-a19c-5308ca800c98","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-human-aldolase-boundary-event","event_type":"observed_relationship","label":"A second proposed glycine-producing route also differs across species.","description":"Human GLY1 contained frameshifting deletions and a premature stop codon; its mRNA was not detected, unlike the transcribed mouse homolog.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3632f314-c57b-5b65-8b73-53c4438b8f3f","slug":"human-gly1-pseudogene","display_name":"Human threonine aldolase / GLY1 pseudogene","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f2b2e71c-d8e4-559f-b24b-fdedb5e9cb80","slug":"human-functional-gly1-route","display_name":"Functional canonical human GLY1 threonine-aldolase route","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Comparative human and mouse gene characterization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This does not exclude all possible promiscuous enzyme activity; it rejects treating canonical human GLY1 as an established active enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A second proposed glycine-producing route also differs across species.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mice have a transcribed L-threonine aldolase/GLY1 gene, but the human GLY1 gene is a non-processed pseudogene. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15757516/ · DOI 10.1186/1471-2164-6-32","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ea13568c-0129-5be6-af25-76e005093745","evidence_kind":"source_excerpt","locator":"Lines 282-288","start_line":282,"end_line":288,"excerpt":"## l-threonine-human-aldolase-boundary\nA second proposed glycine-producing route also differs across species.\nHuman GLY1 contained frameshifting deletions and a premature stop codon; its mRNA was not detected, unlike the transcribed mouse homolog.\nModel: Comparative human and mouse gene characterization.\nLimitations: This does not exclude all possible promiscuous enzyme activity; it rejects treating canonical human GLY1 as an established active enzyme.\nEvidence access: Primary abstract\nMice have a transcribed L-threonine aldolase/GLY1 gene, but the human GLY1 gene is a non-processed pseudogene. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15757516/ · DOI 10.1186/1471-2164-6-32","model_system":"Comparative human and mouse gene characterization.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3365aed939d9f567bd098449c7d67c7c00fb6c9ba163dcf34dc7cf6081f324fb","revision_id":"10a7b648-8dd7-5d4e-b618-c560a6e2d3c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}