{"id":"ab646262-8ac5-5dd4-82f5-65652670e737","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-thiol-loss","predicate":"reduces_tested","statement":"Adding cucurbitacin B rapidly lowered measured protein-thiol levels in human NSCLC cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"6a8e1eba-ebf9-55c5-aa4f-b253e865756c","mechanism_event_label":"Reactive protein groups decreased after exposure.","subject":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"object":{"id":"0e00b97a-0517-52ce-ba03-38ab0024e413","slug":"human-nsclc-protein-thiol-pool","display_name":"Human NSCLC cellular protein-thiol pool","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6a8e1eba-ebf9-55c5-aa4f-b253e865756c","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-thiol-loss-event","event_type":"observed_relationship","label":"Reactive protein groups decreased after exposure.","description":"Adding cucurbitacin B rapidly lowered measured protein-thiol levels in human NSCLC cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0e00b97a-0517-52ce-ba03-38ab0024e413","slug":"human-nsclc-protein-thiol-pool","display_name":"Human NSCLC cellular protein-thiol pool","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human H1299, A549, HCC827 and H661 study panel; exact assay allocation and dose not resolved from the abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A cellular thiol measurement is not evidence of whole-body cysteine deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Reactive protein groups decreased after exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin B potently suppresses non-small-cell lung cancer growth: identification of intracellular thiols as critical targets. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23340170/ · DOI 10.1016/j.canlet.2013.01.008","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c333520d-361f-53e5-aad7-77a37c521c25","evidence_kind":"source_excerpt","locator":"Lines 124-130","start_line":124,"end_line":130,"excerpt":"## cucurbitacin-b-thiol-loss\nReactive protein groups decreased after exposure.\nAdding cucurbitacin B rapidly lowered measured protein-thiol levels in human NSCLC cells.\nModel: Human H1299, A549, HCC827 and H661 study panel; exact assay allocation and dose not resolved from the abstract.\nLimitations: A cellular thiol measurement is not evidence of whole-body cysteine deficiency.\nEvidence access: Primary abstract\nCucurbitacin B potently suppresses non-small-cell lung cancer growth: identification of intracellular thiols as critical targets. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23340170/ · DOI 10.1016/j.canlet.2013.01.008","model_system":"Human H1299, A549, HCC827 and H661 study panel; exact assay allocation and dose not resolved from the abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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