{"id":"ab4414c8-69bb-59f8-8954-f5f43a9ef5bb","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-got2-plp","predicate":"binds-to","statement":"Cryo-EM of human liver mitochondria resolved PLP in GOT2, with residues from both subunits forming its binding pocket.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"29285cef-00db-5f26-b5b0-6e183c4ad91d","mechanism_event_label":"The mitochondrial aspartate enzyme also has a B6-binding catalytic site.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"c52a2a79-ca29-5b9c-b8bb-f8481d407841","slug":"got2","display_name":"Human mitochondrial aspartate aminotransferase / GOT2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"29285cef-00db-5f26-b5b0-6e183c4ad91d","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-got2-plp-event","event_type":"biochemical_relationship","label":"The mitochondrial aspartate enzyme also has a B6-binding catalytic site.","description":"Cryo-EM of human liver mitochondria resolved PLP in GOT2, with residues from both subunits forming its binding pocket.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"c52a2a79-ca29-5b9c-b8bb-f8481d407841","slug":"got2","display_name":"Human mitochondrial aspartate aminotransferase / GOT2","entity_type_key":"protein"},"role":"enzyme dimer","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"resolved ligand","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human liver mitochondrial lysate cryo-EM","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structure demonstrates binding; it does not measure the response to B6 restriction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The mitochondrial aspartate enzyme also has a B6-binding catalytic site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-got2-2023] High-Resolution Structural Proteomics of Mitochondria Using the 'Build and Retrieve' Methodology. (2023). https://pmc.ncbi.nlm.nih.gov/articles/PMC10709515/ DOI: 10.1016/j.mcpro.2023.100666","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human liver mitochondrial lysate","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9bdd27f6-c489-567d-bb5f-eb8c4cf955b2","evidence_kind":"source_excerpt","locator":"Lines 765-774","start_line":765,"end_line":774,"excerpt":"### b6-met-got2-plp\nCryo-EM of human liver mitochondria resolved PLP in GOT2, with residues from both subunits forming its binding pocket.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The mitochondrial aspartate enzyme also has a B6-binding catalytic site.\norganism: Homo sapiens\ntissue_or_cell_type: Human liver mitochondrial lysate\nexperimental_model: Human liver mitochondrial lysate cryo-EM\nlimitations: Structure demonstrates binding; it does not measure the response to B6 restriction.\n[b6-got2-2023] High-Resolution Structural Proteomics of Mitochondria Using the 'Build and Retrieve' Methodology. (2023). https://pmc.ncbi.nlm.nih.gov/articles/PMC10709515/ DOI: 10.1016/j.mcpro.2023.100666","model_system":"Human liver mitochondrial lysate cryo-EM","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-got2-2023] High-Resolution Structural Proteomics of Mitochondria Using the 'Build and Retrieve' Methodology. (2023). https://pmc.ncbi.nlm.nih.gov/articles/PMC10709515/ DOI: 10.1016/j.mcpro.2023.100666","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}