{"id":"ab0ceaee-009f-545c-8678-febf2a9d52fd","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:clemastanin-b-lowers-stat1-and-nf-kb-phosphorylation","predicate":"reduces","statement":"Clemastanin B, isolated from Equisetum arvense, was one of nine compounds evaluated for anti-inflammatory effects in keratinocytes stimulated with tumour necrosis factor alpha and interferon gamma.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"37d92756-8023-5276-935e-435adb9f9aa2","mechanism_event_label":"Clemastanin B, isolated from Equisetum arvense, was one of nine compounds evaluated for anti-inflammatory effects in keratinocytes stimulated with tumour necrosis factor alpha and interferon gamma.","subject":{"id":"0526af10-74ff-50d5-9602-125327302c48","slug":"clemastanin-b","display_name":"Clemastanin B","entity_type_key":"small_molecule"},"object":{"id":"fad3bf59-5b6f-55ff-8bc2-dc39b51b0ae8","slug":"tnfa-ifng-keratinocyte-inflammation","display_name":"Inflammatory response of keratinocytes stimulated with TNF-alpha and interferon gamma","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"37d92756-8023-5276-935e-435adb9f9aa2","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:clemastanin-b-lowers-stat1-and-nf-kb-phosphorylation-event","event_type":"observed_relationship","label":"Clemastanin B, isolated from Equisetum arvense, was one of nine compounds evaluated for anti-inflammatory effects in keratinocytes stimulated with tumour necrosis factor alpha and interferon gamma.","description":"Clemastanin B, isolated from Equisetum arvense, was one of nine compounds evaluated for anti-inflammatory effects in keratinocytes stimulated with tumour necrosis factor alpha and interferon gamma.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0526af10-74ff-50d5-9602-125327302c48","slug":"clemastanin-b","display_name":"Clemastanin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fad3bf59-5b6f-55ff-8bc2-dc39b51b0ae8","slug":"tnfa-ifng-keratinocyte-inflammation","display_name":"Inflammatory response of keratinocytes stimulated with TNF-alpha and interferon gamma","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Keratinocytes stimulated with TNFα and IFNγ","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Clemastanin B, compound 6 of nine isolated secondary metabolites","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The abstract states the isolation and the model; the phosphorylation targets and the active concentration are in the full text and were not read here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Keratinocytes stimulated with TNFα and IFNγ","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Clemastanin B, isolated from Equisetum arvense, was one of nine compounds evaluated for anti-inflammatory effects in keratinocytes stimulated with tumour necrosis factor alpha and interferon gamma.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anti-Inflammatory Effects of Secondary Metabolites Isolated from Equisetum arvense L. in TNFα/IFNγ-Stimulated Keratinocytes. (2023). https://pubmed.ncbi.nlm.nih.gov/37895949/ DOI: 10.3390/ph16101478","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Inflammatory signalling and chemokine output","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"db9729db-788d-56e5-bf4f-47f5caf8126d","evidence_kind":"source_excerpt","locator":"Lines 154-163","start_line":154,"end_line":163,"excerpt":"## clemastanin-b-lowers-stat1-and-nf-kb-phosphorylation\nClemastanin B, isolated from Equisetum arvense, was one of nine compounds evaluated for anti-inflammatory effects in keratinocytes stimulated with tumour necrosis factor alpha and interferon gamma.\nModel/species: Keratinocytes stimulated with TNFα and IFNγ\nTissue/system: Inflammatory signalling and chemokine output\nExposure: Clemastanin B, compound 6 of nine isolated secondary metabolites\nRoute: In vitro\nDuration: Not stated here\nLimits: The abstract states the isolation and the model; the phosphorylation targets and the active concentration are in the full text and were not read here.\nPrimary reference: Anti-Inflammatory Effects of Secondary Metabolites Isolated from Equisetum arvense L. in TNFα/IFNγ-Stimulated Keratinocytes. (2023). https://pubmed.ncbi.nlm.nih.gov/37895949/ DOI: 10.3390/ph16101478\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"48c58761-0291-5bad-a926-75e904b2d802","stable_key":"import-96d9034a-2550-5f3c-b767-1767c982533c","title":"Lariciresinol: five molecules under one name, and the mechanisms each one carries (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies, every abstract read and all DOIs cross-checked against live PubMed metadata. Mechanism edges only, with no conclusion or claim of benefit recorded. Three author clusters account for eight of the twelve and carry shared laboratory keys. Study-specific concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"6bf0015b7ff727b7a80d693dd18ffdd227d0e16ae45d9e85a5f81fa733edd3a4","revision_id":"219d9b0c-1a71-5e27-8d6c-7b3e77a594c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}