{"id":"aac2fe73-e90a-5358-8143-eccd97e78413","stable_key":"research:selenom-supports-leptin-stat3-response","predicate":"supports-phosphorylation","statement":"SELENOM depletion reduced leptin-evoked STAT3 phosphorylation in hypothalamic-cell experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"positive","is_public":true,"mechanism_event_id":"a5f42cd4-c889-5e7e-8942-6f96e2928598","mechanism_event_label":"SELENOM helped these cells respond to leptin.","subject":{"id":"7ba3791b-64d4-5a98-b084-03eef281b738","slug":"selenom","display_name":"SELENOM","entity_type_key":"protein"},"object":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a5f42cd4-c889-5e7e-8942-6f96e2928598","stable_key":"research:selenom-supports-leptin-stat3-response","event_type":"genetic-perturbation","label":"SELENOM helped these cells respond to leptin.","description":"SELENOM depletion reduced leptin-evoked STAT3 phosphorylation in hypothalamic-cell experiments.","status":"active","compartment":null,"participants":[{"entity":{"id":"7ba3791b-64d4-5a98-b084-03eef281b738","slug":"selenom","display_name":"SELENOM","entity_type_key":"protein"},"role":"perturbed-protein","stoichiometry":null,"state_label":"depleted","sequence_order":0,"notes":""},{"entity":{"id":"17a76361-15de-5b83-8168-f36f8cc8cc3d","slug":"leptin","display_name":"LEP","entity_type_key":"protein"},"role":"stimulus","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"role":"signaling-readout","stoichiometry":null,"state_label":"phosphorylated","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"mHypoE-44 hypothalamic cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Knockdown and knockout","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not demonstrate direct enzymatic action on STAT3.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mouse","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d9714232-fb65-5f68-b651-9c5d376bbdb0","evidence_kind":"curated_literature_summary","locator":"lines 786-796","start_line":786,"end_line":796,"excerpt":"## selenom-supports-leptin-stat3-response\n\nSELENOM helped these cells respond to leptin.\n\nSELENOM depletion reduced leptin-evoked STAT3 phosphorylation in hypothalamic-cell experiments.\n\nOrganism: mouse\nCell type: mHypoE-44 hypothalamic cells\nExperimental model: Knockdown and knockout\nLimitations: Does not demonstrate direct enzymatic action on STAT3.\nPrimary reference: [Selenoprotein M Promotes Hypothalamic Leptin Signaling and Thioredoxin Antioxidant Activity](https://pmc.ncbi.nlm.nih.gov/articles/PMC8617589/)","model_system":"Knockdown and knockout","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"hypothalamic-signaling","plain_language":"SELENOM helped these cells respond to leptin.","evidence_scope":"direct_experimental","papers":[{"key":"gong-2019-selenom-leptin","title":"Selenoprotein M Promotes Hypothalamic Leptin Signaling and Thioredoxin Antioxidant Activity","url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8617589/","doi":"10.1089/ars.2018.7594","year":2019,"model":"Mouse knockout and mHypoE-44 hypothalamic-cell perturbations","summary":"Tests SELENOM effects on leptin-responsive signaling and thioredoxin activity."}]}}