{"id":"aa829a9e-be15-54fa-b016-0ac803d1b086","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-rat-direct-plp","predicate":"undergoes","statement":"Hydrolysis-protected rat intestinal sacs showed serosal PLP appearance; the authors interpreted accompanying venous data as supporting some direct phosphorylated-PLP absorption.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8386190b-8d21-523a-be2c-dad9bef1baac","mechanism_event_label":"A rat experiment suggests PLP can sometimes cross intact.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"f495dbab-3e6b-5ede-9ad2-bdfd0cbadd6d","slug":"intestinal-plp-absorption","display_name":"Intestinal PLP absorption","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8386190b-8d21-523a-be2c-dad9bef1baac","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-rat-direct-plp-event","event_type":"biochemical_relationship","label":"A rat experiment suggests PLP can sometimes cross intact.","description":"Hydrolysis-protected rat intestinal sacs showed serosal PLP appearance; the authors interpreted accompanying venous data as supporting some direct phosphorylated-PLP absorption.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f495dbab-3e6b-5ede-9ad2-bdfd0cbadd6d","slug":"intestinal-plp-absorption","display_name":"Intestinal PLP absorption","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_location","value_text":"Indexed abstract: hydrolysis-protected sac experiments and in-situ sampling","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat everted intestinal sacs and mesenteric venous sampling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Physiological-range 0.1-1 micromolar PLP and additional concentration-response experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish the quantitative contribution in humans or exclude hydrolysis/rephosphorylation in every preparation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A rat experiment suggests PLP can sometimes cross intact.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[morita1988] Intestinal absorption of pyridoxal 5'-phosphate at physiological levels in rats. (1988). https://pubmed.ncbi.nlm.nih.gov/3244043/ DOI: 10.3177/jnsv.34.553","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Small intestine","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0c7e1643-6abf-5a99-90d7-eb37c6665b93","evidence_kind":"source_excerpt","locator":"Lines 98-109","start_line":98,"end_line":109,"excerpt":"### b6-transport-rat-direct-plp\nHydrolysis-protected rat intestinal sacs showed serosal PLP appearance; the authors interpreted accompanying venous data as supporting some direct phosphorylated-PLP absorption.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A rat experiment suggests PLP can sometimes cross intact.\norganism: Rattus norvegicus\ntissue_or_cell_type: Small intestine\nexperimental_model: Rat everted intestinal sacs and mesenteric venous sampling.\nlimitations: Does not establish the quantitative contribution in humans or exclude hydrolysis/rephosphorylation in every preparation.\nexposure: Physiological-range 0.1-1 micromolar PLP and additional concentration-response experiments.\nevidence_location: Indexed abstract: hydrolysis-protected sac experiments and in-situ sampling\n[morita1988] Intestinal absorption of pyridoxal 5'-phosphate at physiological levels in rats. 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