{"id":"aa4ef257-7da9-5da1-9809-fc3c7c1e7e7d","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-neurospheres","predicate":"promotes","statement":"Sustained theanine increased neurosphere growth and MTT-reducing activity in rat progenitor cultures.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5349f1c3-1f1c-56f9-a4ad-e0899d5e9e9a","mechanism_event_label":"The experimental progenitor clusters grew; this is not a clinical brain-growth claim.","subject":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"object":{"id":"e8db8ea4-3fe8-582d-b8ea-703a1d3c24d0","slug":"rat-neurosphere-growth","display_name":"Rat neural-progenitor neurosphere growth","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5349f1c3-1f1c-56f9-a4ad-e0899d5e9e9a","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-neurospheres-event","event_type":"biochemical_relationship","label":"The experimental progenitor clusters grew; this is not a clinical brain-growth claim.","description":"Sustained theanine increased neurosphere growth and MTT-reducing activity in rat progenitor cultures.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e8db8ea4-3fe8-582d-b8ea-703a1d3c24d0","slug":"rat-neurosphere-growth","display_name":"Rat neural-progenitor neurosphere growth","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/theanine-research/25957749.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7ebc4fc37f26e19c2d7fb13442e263182e628beda7286839f7d0de0906d59f8e\", \"start_char\": 0, \"end_char\": 1727, \"text_sha256\": \"7ebc4fc37f26e19c2d7fb13442e263182e628beda7286839f7d0de0906d59f8e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Sustained exposure, neurospheres and promoter-reporter experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Theanine 1-100 micromolar; sustained culture exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Immature cells differ from mature neurons/astroglia. Expression changes are not demonstrated neurogenesis in adult humans; reporter experiments do not prove every causal link.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Theanine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Embryonic rat neural progenitors; mouse P19 reporter cells in separate assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The experimental progenitor clusters grew; this is not a clinical brain-growth claim.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[theanine-p25957749] Upregulation of Slc38a1 Gene Along with Promotion of Neurosphere Growth and Subsequent Neuronal Specification in Undifferentiated Neural Progenitor Cells Exposed to Theanine. (2016). https://pubmed.ncbi.nlm.nih.gov/25957749/ DOI: 10.1007/s11064-015-1591-4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Progenitor proliferation and lineage commitment","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bf5ce1a5-3207-5914-add9-9639d7a16dcc","evidence_kind":"source_excerpt","locator":"Lines 341-352","start_line":341,"end_line":352,"excerpt":"### theanine-neurospheres\nSustained theanine increased neurosphere growth and MTT-reducing activity in rat progenitor cultures.\nCondition category: normal\nnutrient_topic: L-Theanine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The experimental progenitor clusters grew; this is not a clinical brain-growth claim.\norganism: Embryonic rat neural progenitors; mouse P19 reporter cells in separate assays\ntissue_or_cell_type: Progenitor proliferation and lineage commitment\nexperimental_model: Sustained exposure, neurospheres and promoter-reporter experiments\nlimitations: Immature cells differ from mature neurons/astroglia. Expression changes are not demonstrated neurogenesis in adult humans; reporter experiments do not prove every causal link.\nexposure: Theanine 1-100 micromolar; sustained culture exposure\nevidence_span: {\"source_cache\": \"artifacts/theanine-research/25957749.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7ebc4fc37f26e19c2d7fb13442e263182e628beda7286839f7d0de0906d59f8e\", \"start_char\": 0, \"end_char\": 1727, \"text_sha256\": \"7ebc4fc37f26e19c2d7fb13442e263182e628beda7286839f7d0de0906d59f8e\"}\n[theanine-p25957749] Upregulation of Slc38a1 Gene Along with Promotion of Neurosphere Growth and Subsequent Neuronal Specification in Undifferentiated Neural Progenitor Cells Exposed to Theanine. 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