{"id":"aa4ef139-9c03-5365-892b-9a4653d36dfe","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-primary-tumor-null","predicate":"does_not_reduce_in_model","statement":"Primary tumor growth was unaffected by A39 loss in the reported comparison.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"31a7cc69-714c-55b8-833a-bd908e17578e","mechanism_event_label":"Early colonization and growth of the original tumor were distinct endpoints.","subject":{"id":"f3806ff7-01fc-5499-9956-0df1a027d2ac","slug":"slc25a39-knockout-cells","display_name":"SLC25A39-knockout mammalian cells","entity_type_key":"protein_state"},"object":{"id":"0e427ce4-9297-58fb-b798-5b6433ca1cc8","slug":"breast-cancer-primary-growth-a39","display_name":"Primary tumor growth in the SLC25A39 breast-cancer study","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"31a7cc69-714c-55b8-833a-bd908e17578e","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-primary-tumor-null-event","event_type":"biochemical_relationship","label":"Early colonization and growth of the original tumor were distinct endpoints.","description":"Primary tumor growth was unaffected by A39 loss in the reported comparison.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f3806ff7-01fc-5499-9956-0df1a027d2ac","slug":"slc25a39-knockout-cells","display_name":"SLC25A39-knockout mammalian cells","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0e427ce4-9297-58fb-b798-5b6433ca1cc8","slug":"breast-cancer-primary-growth-a39","display_name":"Primary tumor growth in the SLC25A39 breast-cancer study","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/glutathione-research/40736010.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"6c5baf25f027a65c991f7d45f87238cad1e474e7e910dceb32aa78a108e6d29e\", \"start_char\": 0, \"end_char\": 1513, \"text_sha256\": \"6c5baf25f027a65c991f7d45f87238cad1e474e7e910dceb32aa78a108e6d29e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Genetic screens, cancer models and patient-derived xenografts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"SLC25A39 loss and ATF4 CRISPR activation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cancer-specific mechanism; no inference that dietary or supplemental GSH causes metastasis in people.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glutathione research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Experimental breast-cancer cells and mouse xenografts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Early colonization and growth of the original tumor were distinct endpoints.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[glutathione-p40736010] Mitochondrial Glutathione Import Enables Breast Cancer Metastasis via Integrated Stress Response Signaling. (2025). https://pubmed.ncbi.nlm.nih.gov/40736010/ DOI: 10.1158/2159-8290.cd-24-1556","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Early lung metastatic colonization","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a3d76c67-c35e-5a22-9b28-47e91b73f7fd","evidence_kind":"source_excerpt","locator":"Lines 1334-1345","start_line":1334,"end_line":1345,"excerpt":"### glutathione-primary-tumor-null\nPrimary tumor growth was unaffected by A39 loss in the reported comparison.\nCondition category: normal\nnutrient_topic: Glutathione research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Early colonization and growth of the original tumor were distinct endpoints.\norganism: Experimental breast-cancer cells and mouse xenografts\ntissue_or_cell_type: Early lung metastatic colonization\nexperimental_model: Genetic screens, cancer models and patient-derived xenografts\nlimitations: Cancer-specific mechanism; no inference that dietary or supplemental GSH causes metastasis in people.\nexposure: SLC25A39 loss and ATF4 CRISPR activation\nevidence_span: {\"source_cache\": \"artifacts/glutathione-research/40736010.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"6c5baf25f027a65c991f7d45f87238cad1e474e7e910dceb32aa78a108e6d29e\", \"start_char\": 0, \"end_char\": 1513, \"text_sha256\": \"6c5baf25f027a65c991f7d45f87238cad1e474e7e910dceb32aa78a108e6d29e\"}\n[glutathione-p40736010] Mitochondrial Glutathione Import Enables Breast Cancer Metastasis via Integrated Stress Response Signaling. (2025). https://pubmed.ncbi.nlm.nih.gov/40736010/ DOI: 10.1158/2159-8290.cd-24-1556","model_system":"Genetic screens, cancer models and patient-derived xenografts","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [glutathione-p40736010] Mitochondrial Glutathione Import Enables Breast Cancer Metastasis via Integrated Stress Response Signaling. (2025). https://pubmed.ncbi.nlm.nih.gov/40736010/ DOI: 10.1158/2159-8290.cd-24-1556","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9b7417fd-059d-5683-b452-71d1e3cf82d0","stable_key":"import-db0fc92e-b5ef-5667-a4c5-3ef257edbc9b","title":"Glutathione: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2a5740c4a7e770aafe883fea47909b18bf5e174f211749935130a50f9dea2753","revision_id":"a64016dd-4b72-5954-a72d-0e61de854073","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}