{"id":"a9a9c524-6d37-5207-aaa8-abe5bc94407a","stable_key":"3d41d18a-e13a-59a0-a081-55bfcdd43e55:ceylon-bark-amylase","predicate":"inhibits","statement":"The bark extracts inhibited bacterial alpha-amylase with reported IC50 values about 214–215 micrograms/mL.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"339ef42c-2c61-5c68-8c1d-a3146ea6fa0e","mechanism_event_label":"The tested enzyme was bacterial, so this is not direct evidence for human pancreatic amylase inhibition.","subject":{"id":"3b64a7a0-b1a9-5c7d-8f2c-2c8f2420ead3","slug":"ceylon-bark-solvent-extracts-2017","display_name":"Authenticated Ceylon bark ethanol or DCM:methanol extracts in 2017","entity_type_key":"chemical_species"},"object":{"id":"0432e382-f1ae-5b08-bb9c-c9fc42f390d1","slug":"bacillus-amyloliquefaciens-alpha-amylase","display_name":"Bacillus amyloliquefaciens alpha-amylase preparation","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"339ef42c-2c61-5c68-8c1d-a3146ea6fa0e","stable_key":"3d41d18a-e13a-59a0-a081-55bfcdd43e55:ceylon-bark-amylase-event","event_type":"biochemical_relationship","label":"The tested enzyme was bacterial, so this is not direct evidence for human pancreatic amylase inhibition.","description":"The bark extracts inhibited bacterial alpha-amylase with reported IC50 values about 214–215 micrograms/mL.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3b64a7a0-b1a9-5c7d-8f2c-2c8f2420ead3","slug":"ceylon-bark-solvent-extracts-2017","display_name":"Authenticated Ceylon bark ethanol or DCM:methanol extracts in 2017","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0432e382-f1ae-5b08-bb9c-c9fc42f390d1","slug":"bacillus-amyloliquefaciens-alpha-amylase","display_name":"Bacillus amyloliquefaciens alpha-amylase preparation","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ceylon-research/28951761.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1620e214c93099dbadb647929446e018afe60ad1f234704a0eab6fcb154ea5d7\", \"start_char\": 0, \"end_char\": 1878, \"text_sha256\": \"1620e214c93099dbadb647929446e018afe60ad1f234704a0eab6fcb154ea5d7\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Authenticated bark/leaf extract enzyme and BSA glycation assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Ethanol or 1:1 DCM:methanol extracts; microgram/mL assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Enzyme species were resolved from methods; albumin fluorescence is a model endpoint, not proof of reversing human tissue AGE damage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ceylon","display_name":"Ceylon cinnamon / Cinnamomum verum bark preparations","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"B. amyloliquefaciens amylase; horse BChE; bovine albumin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The tested enzyme was bacterial, so this is not direct evidence for human pancreatic amylase inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ceylon-p28951761] Antiamylase, Anticholinesterases, Antiglycation, and Glycation Reversing Potential of Bark and Leaf of Ceylon Cinnamon (Cinnamomum zeylanicum Blume) In Vitro. (2017). https://pubmed.ncbi.nlm.nih.gov/28951761/ DOI: 10.1155/2017/5076029","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free preparations","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fe606a34-f803-562a-a4eb-47d69a89fc7b","evidence_kind":"source_excerpt","locator":"Lines 285-296","start_line":285,"end_line":296,"excerpt":"### ceylon-bark-amylase\nThe bark extracts inhibited bacterial alpha-amylase with reported IC50 values about 214–215 micrograms/mL.\nCondition category: normal\nnutrient_topic: Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The tested enzyme was bacterial, so this is not direct evidence for human pancreatic amylase inhibition.\norganism: B. amyloliquefaciens amylase; horse BChE; bovine albumin\ntissue_or_cell_type: Cell-free preparations\nexperimental_model: Authenticated bark/leaf extract enzyme and BSA glycation assays\nlimitations: Enzyme species were resolved from methods; albumin fluorescence is a model endpoint, not proof of reversing human tissue AGE damage.\nexposure: Ethanol or 1:1 DCM:methanol extracts; microgram/mL assays\nevidence_span: {\"source_cache\": \"artifacts/ceylon-research/28951761.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1620e214c93099dbadb647929446e018afe60ad1f234704a0eab6fcb154ea5d7\", \"start_char\": 0, \"end_char\": 1878, \"text_sha256\": \"1620e214c93099dbadb647929446e018afe60ad1f234704a0eab6fcb154ea5d7\"}\n[ceylon-p28951761] Antiamylase, Anticholinesterases, Antiglycation, and Glycation Reversing Potential of Bark and Leaf of Ceylon Cinnamon (Cinnamomum zeylanicum Blume) In Vitro. 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