{"id":"a979a7f5-1bcd-52c0-b2f8-33bed0379cb8","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-rat-calcitriol-conversion-preserved","predicate":"depletion_did_not_reduce","statement":"Magnesium depletion did not impair measured calcifediol-to-calcitriol conversion in these rats; isolated renal activity persisted without added Mg.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"53089dfe-3dca-5a3b-8c12-e6f5e9bf6e55","mechanism_event_label":"Low magnesium did not switch off vitamin D activation in this rat experiment.","subject":{"id":"1f0c42f0-eaf3-5b86-966d-7ffc8a84872b","slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"},"object":{"id":"96b29e93-a2d1-56e7-84a3-2c5a61b4ee09","slug":"calcitriol-production","display_name":"Calcitriol production","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"53089dfe-3dca-5a3b-8c12-e6f5e9bf6e55","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-rat-calcitriol-conversion-preserved-event","event_type":"biochemical_relationship","label":"Low magnesium did not switch off vitamin D activation in this rat experiment.","description":"Magnesium depletion did not impair measured calcifediol-to-calcitriol conversion in these rats; isolated renal activity persisted without added Mg.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6bd73bd2-1f0a-5caa-ba69-7ce1b75364bb","slug":"calcifediol","display_name":"Calcifediol / 25-hydroxyvitamin D3","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f5156c24-108f-5746-b114-ed764e79a3f4","slug":"calcitriol","display_name":"Calcitriol","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a1d0238c-7529-5175-b5f9-71dc9bc61ed5","slug":"cyp27b1","display_name":"Vitamin D 1-alpha-hydroxylase / CYP27B1","entity_type_key":"protein"},"role":"enzyme_identity","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1f0c42f0-eaf3-5b86-966d-7ffc8a84872b","slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"96b29e93-a2d1-56e7-84a3-2c5a61b4ee09","slug":"calcitriol-production","display_name":"Calcitriol production","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Magnesium/vitamin D: qualify a universal CYP27B1 shutdown claim.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"In-vivo tracer and isolated mitochondrial assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No-added-Mg buffer does not prove a metal-free preparation. This result cannot be generalized to every species or illness.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Low magnesium did not switch off vitamin D activation in this rat experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mg-carpenter1987] Effect of magnesium depletion on metabolism of 25-hydroxyvitamin D in rats (1987). https://pubmed.ncbi.nlm.nih.gov/3605332/ DOI: 10.1152/ajpendo.1987.253.1.e106","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Rat circulation and isolated kidney mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"335ad544-e48a-5013-b741-c32870a5e2c1","evidence_kind":"source_excerpt","locator":"Lines 1370-1380","start_line":1370,"end_line":1380,"excerpt":"### mg-rat-calcitriol-conversion-preserved\nMagnesium depletion did not impair measured calcifediol-to-calcitriol conversion in these rats; isolated renal activity persisted without added Mg.\nCondition category: nutrient_deficiency\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Low magnesium did not switch off vitamin D activation in this rat experiment.\norganism: Rattus norvegicus\ntissue_or_cell_type: Rat circulation and isolated kidney mitochondria\nexperimental_model: In-vivo tracer and isolated mitochondrial assays.\nlimitations: No-added-Mg buffer does not prove a metal-free preparation. This result cannot be generalized to every species or illness.\ncross_nutrient: Magnesium/vitamin D: qualify a universal CYP27B1 shutdown claim.\n[mg-carpenter1987] Effect of magnesium depletion on metabolism of 25-hydroxyvitamin D in rats (1987). https://pubmed.ncbi.nlm.nih.gov/3605332/ DOI: 10.1152/ajpendo.1987.253.1.e106","model_system":"In-vivo tracer and isolated mitochondrial assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mg-carpenter1987] Effect of magnesium depletion on metabolism of 25-hydroxyvitamin D in rats (1987). https://pubmed.ncbi.nlm.nih.gov/3605332/ DOI: 10.1152/ajpendo.1987.253.1.e106","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}